|
|
| Sonobe,M.H.; Trezena,A.G.; Guilhen,F.B.; Takano,V.L.; Fratelli,F.; Sakauchi,D.; Morais,J.F.; Prado,S.M.A.; Higashi,H.G.. |
| A method for the screening of tetanus and diphtheria antibodies in serum using anatoxin (inactivated toxin) instead of toxin was developed as an alternative to the in vivo toxin neutralization assay based on the toxin-binding inhibition test (TOBI test). In this study, the serum titers (values between 1.0 and 19.5 IU) measured by a modified TOBI test (Modi-TOBI test) and toxin neutralization assays were correlated (P < 0.0001). Titers of tetanus or diphtheria antibodies were evaluated in serum samples from guinea pigs immunized with tetanus toxoid, diphtheria-tetanus or triple vaccine. For the Modi-TOBI test, after blocking the microtiter plates, standard tetanus or diphtheria antitoxin and different concentrations of guinea pig sera were incubated with... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tetanus; Diphtheria; Toxin-binding inhibition test; Toxin neutralization. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000100009 |
| |
