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Use of low density polyethylene membranes for assessment of genotoxicity of PAHs in the Seine River ArchiMer
Vincent-hubert, Francoise; Uher, Emmanuelle; Di Giorgio, Carole; Michel, Cecile; De Meo, Michel; Gourlay-france, Catherine.
The genotoxicity of river water dissolved contaminants is usually estimated after grab sampling of river water. Water contamination can now be obtained with passive samplers that allow a time-integrated sampling of contaminants. Since it was verified that low density polyethylene membranes (LDPE) accumulate labile hydrophobic compounds, their use was proposed as a passive sampler. This study was designed to test the applicability of passive sampling for combined chemical and genotoxicity measurements. The LDPE extracts were tested with the umu test (TA1535/pSK1002 ± S9) and the Ames assay (TA98, TA100 and YG1041 ± S9). We describe here this new protocol and its application in two field studies on four sites of the Seine River. Field LDPE extracts were...
Tipo: Text Palavras-chave: Genotoxicity; River water; Passive samplers; PAHs; Ames assay; Umu test.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00371/48225/48402.pdf
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To what extend the dam dredging can influence the background level of metals in the Rhine River: using chemical and biological long-term monitoring to answer ArchiMer
Lebrun, Jeremie D.; Dufour, Marine; Uher, Emmanuelle; Fabure, Juliette; Mons, Raphael; Charlatchka, Rayna; Gourlay-france, Catherine; Fechner, Lise; Ferrari, Benoit Jd.
Dredging generates remobilisation of sediments contaminated by non-degradable compounds such as metals, to which aquatic organisms can be exposed. This study aims at assessing the environmental impact of sediments remobilised in the Rhine River (France) during the dredging of Marckolsheim dam by pumping/dilution in 2013 on metal speciation and organisms' exposure. The monitoring coupling chemical and biological tools was performed 2 years before dredging operation on 2 sampling sites, upstream and downstream from the discharge of pumping/dilution, to acquire data on the natural variability of labile (DGT as passive samplers), dissolved and particulate concentrations of Cd, Co, Cr, Cu, Ni, Mn, Pb and Zn in Rhine during full hydrological cycles. In parallel,...
Tipo: Text Palavras-chave: Active biomonitoring; Dreissena polymorpha; DGT; Metal bioavailability; Natural variability.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00413/52458/53244.pdf
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Waterborne nickel bioaccumulation in Gammarus pulex: Comparison of mechanistic models and influence of water cationic composition ArchiMer
Lebrun, Jeremie D.; Perret, Marine; Uher, Emmanuelle; Tusseau-vuillemin, Marie-helene; Gourlay-france, Catherine.
The biodynamic and saturation models offer promising lines of enquiry to predict the bioaccumulation of metals by aquatic organisms. However, in order to construct these models, the accumulation strategies have to be defined for each metal/organism couple in controlled conditions. This study aims at modelling the waterborne bioaccumulation of Ni and the influence of the water's geochemical properties on this process in a crustacean that is widely distributed in Europe, Gammarus pulex. In the laboratory, G. pulex was exposed to several Ni concentrations (from 0.001 to 100 mg L(-1)) in aquatic microcosms. Our results show that G. pulex is very tolerant to Ni (LC50(48 h) = 477 mg L(-1) Ni). Time course experiments enabled the construction of a biodynamic...
Tipo: Text Palavras-chave: Biodynamic kinetics; Saturation model; Gammarid; Bioavailability; Water chemistry; Aquatic microcosms.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00041/15217/18005.pdf
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Genotoxicity assessment and detoxification induction in Dreissena polymorpha exposed to benzo[a]pyrene ArchiMer
Chatel, Amelie; Faucet-marquis, Virginie; Perret, Marine; Gourlay-france, Catherine; Uher, Emmanuelle; Pfohl-leszkowicz, Annie; Vincent-hubert, Francoise.
The use of DNA adduct analysis has previously focused on the use of marine organisms for biomonitoring, whereas similar investigations in freshwater organisms are sparse. In that context, we have investigated the relevance of DNA adducts as biomarkers of genotoxicity in the freshwater mussels Dreissena polymorpha. The objective of the present study is to determine the stability of DNA adducts induced by benzo[a]pyrene (B[a]P) in zebra mussels. Mussels were exposed to dissolved B[a]P (10100 mu g/l) for 4 days. Afterwards, mussels were kept in clean water for 28 days and DNA adduct levels were subsequently measured in two different organs, the digestive glands and the gills, using the P-32-postlabelling technique. In parallel, the expression of genes...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00184/29566/27912.pdf
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Essential metal contents in indigenous gammarids related to exposure levels at the river basin scale: Metal-dependent models of bioaccumulation and geochemical correlations ArchiMer
Lebrun, Jeremie D.; Uher, Emmanuelle; Tusseau-vuillemin, Marie-helene; Gourlay-france, Catherine.
Biomonitoring, assumed to be an integrative measurement of the chemical exposure of aquatic organisms, is not straightforward for essential metals because they can be actively regulated by animals. Although increasing bioaccumulation with exposure levels is a crucial endpoint for the development of biomonitors, it is rarely verified in real environments, where the metal concentrations are rather low and vary little. This study was designed at the scale of a river basin to assess the ability of Gammarus pulex indigenous populations to accumulate Cu, Zn and Mn in realistic exposure conditions. During two annual campaigns, water and gammarids were collected at various sites contrasted in terms of physicochemistry and contamination. The results show...
Tipo: Text Palavras-chave: Biomonitor; Metal bioavailability; Gammarus pulex; Copper; Zinc; Manganese.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00180/29154/28656.pdf
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In situ measurement with diffusive gradients in thin films: effect of biofouling in freshwater ArchiMer
Uher, Emmanuelle; Compere, Chantal; Combe, Matthieu; Mazeas, Florence; Gourlay-france, Catherine.
Concerning in situ passive sampler deployment, several technical priorities must be considered. In particular, deployment time must be sufficiently long not only to allow a significant quantity to be accumulated to facilitate analysis but also to ensure that the signal is above the quantification limit and out of the blank influence. Moreover, regarding the diffusive gradient in thin films (DGT) technique, deployment time must also be sufficiently long (at least 5 days) to avoid the interactions of the solutes with the material diffusion layer of the DGT and for the steady state to be reached in the gel. However, biofouling occurs in situ and modifies the surface of the samplers. In this article, we propose a kinetic model which highlights the biofouling...
Tipo: Text Palavras-chave: DGT; Metals; Biofouling; Seine River; Field deployment; Passive sampler.
Ano: 2017 URL: http://archimer.ifremer.fr/doc/00382/49330/49759.pdf
Registros recuperados: 6
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