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New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse Biol. Res.
FAROUDI,MUSTAPHA; ZARU,ROSSANA; FAVIER,BENOIT; VALITUTTI,SALVATORE.
Three innovative and complementary morphological approaches were employed to study the T cell/antigen presenting cell (APC) interaction: (i) high resolution three-dimensional confocal microscopy of the T cell-APC contact site; (ii) time lapse video recording in living T cells of [Ca2+]I and changes in distribution of various GFP fusion proteins with TCR/CD3-zetacomplex associated- and other signaling components; (iii) measurement of lateral TCR mobility and that of recruited signaling components using techniques based on fluorescence recovery after photo-bleaching. These approaches were combined with biochemical and functional experiments to investigate two principal issues: (A) Recruitment and the three-dimensional arrangement of receptors and signaling...
Tipo: Journal article Palavras-chave: T cell antigen receptor; Immunological synapse; T cell antigen receptor; Signal transduction.
Ano: 2002 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200004
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