The short time degradation of crude oil "Arabe leger" was followed through several seasons using different biological and chemical parameters. Regular sampling allowed to measure total epifluorescent microflora, heterotrophic and hydrocarbon microflora, heterotrophic potentials with super(14)C glucose, glutamate and naphthalene. Some experiments concern taxonomic control of microflora development (API 20B). Shifts in M.P.N. numbers of total and specialized heterotrophic bacteria and quick responses in heterotrophic potentials appear in these cold area (8 degree C max.). Incubation in natural ecosystems allows to reduce the confinement effect observed in closed systems. |