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Bioconversion of agro-industrial wastes for the production of fibrinolytic enzyme from Bacillus halodurans IND18: Purification and biochemical characterization Electron. J. Biotechnol.
Vijayaraghavan,Ponnuswamy; Prakash Vincenta,Samuel Gnana; Valan Arasu,Mariadhas; Al-Dhabi,Naif Abdullah.
Background: Agro-wastes were used for the production of fibrinolytic enzyme in solid-state fermentation. The process parameters were optimized to enhance the production of fibrinolytic enzyme from Bacillus halodurans IND18 by statistical approach. The fibrinolytic enzyme was purified, and the properties were studied. Results: A two-level full factorial design was used to screen the significant factors. The factors such as moisture, pH, and peptone were significantly affected enzyme production and these three factors were selected for further optimization using central composite design. The optimum medium for fibrinolytic enzyme production was wheat bran medium containing 1% peptone and 80% moisture with pH 8.32. Under these optimized conditions, the...
Tipo: Journal article Palavras-chave: Agro-residues; Solid-state fermentation; Statistical approach; Thrombolytic agent.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000200001
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Statistical optimization of thermo-alkali stable xylanase production from Bacillus tequilensis strain ARMATI Electron. J. Biotechnol.
Khusro,Ameer; Kannan Kaliyan,Barathi; Abdullah Al-Dhabi,Naif; Valan Arasu,Mariadhas; Agastian,Paul.
Background: Xylanase from bacteria finds use in prebleaching process and bioconversion of lignocelluloses into feedstocks. The xylanolytic enzyme brings about the hydrolysis of complex biomolecules into simple monomer units. This study aims to optimize the cellulase-free xylanase production and cell biomass of Bacillus tequilensis strain ARMATI using response surface methodology (RSM). Results: Statistical screening of medium constituents and the physical factors affecting xylanase and biomass yield of the isolate were optimized by RSM using central composite design at N = 30, namely 30 experimental runs with 4 independent variables. The central composite design showed 3.7 fold and 1.5 fold increased xylanase production and biomass yield of the isolate...
Tipo: Journal article Palavras-chave: Bacillus tequilensis; Biomass; Central composite design; RSM; Xylanase.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000400003
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