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| Valiyari,Samira; Mahdian,Reza; Salami,Mona; Oloomi,Mana; Golshani,Maryam; Shokrgozar,Mohammad Ali; Bouzari,Saeid. |
| ABSTRACT Interleukin-24 (IL-24) is a novel tumor-suppressor gene that has different alternative splice isoforms. It has been shown that new smallest isoform of human IL-24 gene, lacking three exons, induces higher levels of cytotoxicity than all the isoforms, indicating shortest isoform of IL-24 may be a new promising anti-cancer agent. In this study, we aimed to provide a reproducible method for recombinant production of the smallest isoform of IL-24 (sIL-24). The Structure of sIL-24 was analyzed using bioinformatics tools (I-TASSER, Prosa, RAMPAGE and SPDBV version 4.1). The DNA sequence encoding sIL-24 was chemically synthesized and sub-cloned into the pET-32a (+) vector for further protein expression in Escherichia coli BL21 (DE3) strain. Upon IPTG... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Smallest isoform of IL-24; Thioredoxin; Recombinant production; Anti-cancer agent. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100307 |
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