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Glutamate dehydrogenase and Na+-K+ ATPase expression and growth response of Litopenaeus vannamei to different salinities and dietary protein levels ArchiMer
Li, Erchao; Arena, Leticia; Lizama, Gabriel; Gaxiola, Gabriela; Cuzon, Gerard; Rosas, Carlos; Chen, Liqiao; Van Wormhoudt, Alain.
Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture. The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp (L. vannamei) were investigated. This involved an examination of growth performance, glutamate dehydrogenase (GDH) and Na+-K+ ATPase mRNA expression,, and GDH activity in muscles and gills. Three experimental diets were formulated, containing 25%, 40%, and 50% dietary protein, and fed to the shrimp at a salinity of 25. After 20 days, no significant difference was observed in weight gain, though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels. Subsequently, shrimp fed diets with 25% and 50% dietary...
Tipo: Text Palavras-chave: Litopenaeus vannamei; Gene expression; Glutamate dehydrogenase; Na+-K+ ATPase; Protein; Salinity.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14261/11692.pdf
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Caracterization de las trypsinas y amilasas de Penaeus vannamei (crustacea decapoda) : adaptacion a la composicion deln regimen alimenticio ArchiMer
Van Wormhoudt, Alain; Le Moullac, Gilles; Klein, Birgit; Sellos, Daniel.
Las tripsinas, entre las proteasas, y las amilasas, entre las carbohidrasas, son las enzimas más activas en Penaeus vannamei, un camarón de interés comercial, especialmente en Latinoamérica. Estas enzimas y sus cDNAS fueron caracterizados. En lo que respecta a las tripsinas, se reconocieron cinco isoformas y se analizaron sus cDNAs correspondientes, los cuales codifican una preproenzima de 225 aminoácidos conteniendo un supuesto precursor peptídico de 14 residuos y una secuencia señal altamente hidrofobica de 14 aminoácidos. Dentro de la secuencia del zimogeno, se detectaron algunos sitios autocatalíticos (Int. J. Biochem. Cell Biol. 1996, 28, 551-563). Con respecto a las amilasas, dos isoformas fueron purificadas y caracterizadas; se hizo una selección en...
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Ano: 1999 URL: http://archimer.ifremer.fr/doc/00176/28726/27183.pdf
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Phylogeography of the reef-building polychaetes of the genus Phragmatopoma in the western Atlantic Region ArchiMer
Nunes, Flavia; Van Wormhoudt, Alain; Faroni-perez, Larisse; Fournier, Jerome.
Aim To verify the synonymy of the reef-building polychaete Phragmatopoma caudata (described for the Caribbean) and Phragmatopoma lapidosa (described for Brazil) using molecular data. To evaluate the patterns of genetic diversity and connectivity among populations from Florida to South Brazil. Location Intertidal zone in the western Atlantic biogeographical Region: Brazil, eastern Caribbean and Florida (USA). Methods DNA sequence data from one mitochondrial (cox-1) and one nuclear ribosomal (ITS-1) loci were obtained from 11 populations of P. caudata spanning the coasts of Brazil, eastern Caribbean and Florida. Phylogenetic relationships among populations of P. caudata and other members of the genus were inferred by Bayesian methods. Population...
Tipo: Text Palavras-chave: Biogeographical barrier; Brazilian Province; Caribbean Province; Connectivity; Larval dispersal; Phragmatopoma caudata; Phylogenetics; Phylogeography; Polychaete reef; Western Atlantic Region.
Ano: 2017 URL: https://archimer.ifremer.fr/doc/00363/47376/47710.pdf
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