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| Vaulot, Daniel; Lepere, Cecile; Toulza, Eve; De La Iglesia, Rodrigo; Poulain, Julie; Gaboyer, Frederic; Moreau, Herve; Vandepoele, Klaas; Ulloa, Osvaldo; Gavory, Frederick; Piganeau, Gwenael. |
| Among small photosynthetic eukaryotes that play a key role in oceanic food webs, picoplanktonic Mamiellophyceae such as Bathycoccus, Micromonas, and Ostreococcus are particularly important in coastal regions. By using a combination of cell sorting by flow cytometry, whole genome amplification (WGA), and 454 pyrosequencing, we obtained metagenomic data for two natural picophytoplankton populations from the coastal upwelling waters off central Chile. About 60% of the reads of each sample could be mapped to the genome of Bathycoccus strain from the Mediterranean Sea (RCC1105), representing a total of 9 Mbp (sample T142) and 13 Mbp (sample T149) of non-redundant Bathycoccus genome sequences. WGA did not amplify all regions uniformly, resulting in unequal... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00129/23987/21945.pdf |
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| Yau, Sheree; Krasovec, Marc; Benites, L. Felipe; Rombauts, Stephane; Groussin, Mathieu; Vancaester, Emmelien; Aury, Jean-marc; Derelle, Evelyne; Desdevises, Yves; Escande, Marie-line; Grimsley, Nigel; Guy, Julie; Moreau, Hervé; Sanchez-brosseau, Sophie; Van De Peer, Yves; Vandepoele, Klaas; Gourbiere, Sebastien; Piganeau, Gwenael. |
| Virus-microbe interactions in the ocean are commonly described by “boom and bust” dynamics, whereby a numerically dominant microorganism is lysed and replaced by a virus-resistant one. Here, we isolated a microalga strain and its infective dsDNA virus whose dynamics are characterized instead by parallel growth of both the microalga and the virus. Experimental evolution of clonal lines revealed that this viral production originates from the lysis of a minority of virus-susceptible cells, which are regenerated from resistant cells. Whole-genome sequencing demonstrated that this resistant-susceptible switch involved a large deletion on one chromosome. Mathematical modeling explained how the switch maintains stable microalga-virus population dynamics... |
| Tipo: Text |
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| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00623/73461/72701.pdf |
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