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Fernandes,Carlos Antônio de Carvalho; Miyauchi,Tatimara Maria; Figueiredo,Ana Cristina Silva de; Palhão,Miller Pereira; Varago,Fabiana Cristina; Nogueira,Ester Siqueira Caixeta; Neves,Jairo Pereira; Miyauchi,Tochimara Aparecida. |
The objective of this work was to evaluate the effects of hormonal synchronization protocols, associated or not with follicular development stimulation, on the recovery of oocytes and on in vitro production of Bos indicus and B. taurus embryos, in different seasons. Ultrasound-guided follicular aspirations (n=237) were performed without pre-treatment (G1, control group) and after follicular wave synchronization (G2), or after follicular wave synchronization and follicle growth induction (G3). Bos indicus produced more oocytes and embryos than B. taurus (18.7±0.9 vs. 11.9±0.6 oocytes and 4.8±0.3 vs. 2.1±0.2 embryos). On average, oocyte and embryo yields were higher in G3 than in G2, and both were greater than in G1, which lead to a higher conversion of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bos indicus; Bos taurus; Artificial insemination; Follicle growth induction; Follicular wave synchronization; FSH stimulation. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2014001000813 |
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Vasconcelos,André Belico de; Souza,Patrícia Castanheira de; Varago,Fabiana Cristina; Lagares,Monique de Albuquerque; Santoro,Marcelo Matos. |
The heat conduction microcalorimeter can be used to evaluate the metabolic rates of the sperm cell. Two ejaculates of four stallions were cooled to +5ºC and checked for sperm motility (bright field microscopy), viability (eosin 3%), functional membrane integrity (hyposmotic swelling test), and heat production (microcalorimetry). Glucose and sperm cell concentrations were determined in order to measure the heat outputs resulting from sperm metabolism. Sperm viability, membrane integrity and sperm motility did not differ among the different glucose concentrations tested. Nevertheless, the highest heat output detected by the microcalorimeter was obtained with 6 mM glucose and 10(8) spermatozoa/mL. Since conduction microcalorimetry offered additional... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microcalorimetry; Glucose; Spermatozoa; Sperm metabolism; Cell viability; Equine. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500010 |
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