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Evaluation of the microbial diversity in sequencing batch reactor treating linear alkylbenzene sulfonate under denitrifying and mesophilic conditions using swine sludge as inoculum BABT
Duarte,Iolanda Cristina Silveira; Oliveira,Lorena Lima de; Okada,Dagoberto Yukio; Prado,Pierre Ferreira do; Varesche,Maria Bernadete Amâncio.
The objective of this study was to evaluate the degradation of Linear Alkylbenzene Sulfonate (LAS) in anaerobic sequencing batch reactor (ASBR) under denitrifying conditions using swine sludge as inoculum. The reactor was operated for 104 days with synthetic substrate containing nitrate, and LAS was added later (22 mg/L). Considering the added mass of the LAS, the adsorbed mass in the sludge and discarded along with the effluent, degradation of the surfactant at the end of operation was 87%, removal of chemical oxygen demand was 86% and nitrate was 98%. The bacterial community was evaluated by cutting the bands and sequencing of polymerase chain reaction (PCR) fragments and denaturing gradient gel electrophoresis (DGGE). The sequences obtained were related...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Linear alkylbenzene sulfonate (LAS); Bioreactor; PCR/DGGE; Denitrified.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300326
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Denitrification coupled with methane anoxic oxidation and microbial community involved identification BABT
Cuba,Renata Medici Frayne; Duarte,Iolanda Cristina; Saavedra,Nora Katia; Varesche,Maria Bernadete Amâncio; Foresti,Eugenio.
In this work, the biological denitrification associated with anoxic oxidation of methane and the microbial diversity involved were studied. Kinetic tests for nitrate (NO3-) and nitrite (NO2-) removal and methane uptake were carried out in 100 mL batch reactors incubated in a shaker (40 rpm) at 30 ºC. Denitrificant/methanotrophic biomass was taken from a laboratory scale reactor fed with synthetic nitrified substrates (40 mgN L-1 of NO3- and subsequently NO2-) and methane as carbon source. Results obtained from nitrate removal followed a first order reaction, presenting a kinetic apparent constant (kNO3)) of 0.0577±0.0057d-1. Two notable points of the denitrification rate (0.12gNO3--N g-1 AVS d-1 and 0.07gNO3--N g-1 AVS d-1) were observed in the beginning...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Denitrification; Methylomonas sp; Methane oxidation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000100022
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The Influence of Stirring Speed, Temperature and Initial Nitrogen Concentration on Specific Anammox Activity BABT
Martins,Tiago H.; Souza,Theo S.O.; Varesche,Maria Bernadete Amâncio.
ABSTRACT This study evaluates the influence of initial nitrogen concentration, temperature and stirring speed on specific anammox activity (SAA). The biomass was tested in single batch reactors with different initial nitrogen concentrations (Assay 1) ranging from 60 to 140 mg Ntotal/L in equimolar ratio (NO2--N/NH4+-N) and in another test to 67.3 mg NH4+-N/L and 92.2 mg NO2--N/L (close to anammox stoichiometric ratio). The anammox biomass was also tested in single batch at different temperatures (from 20 to 37° C) to determine the short-term effects on SAA (Assay 2). In the third assay the stirring speed ranged from 50 to 150 rpm in a sequencing batch reactor (SBR) at 37 ºC. SAA was affected by the stoichiometric molar ratio but not by equimolar initial...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SBR; Ammonium; Nitrite; Specific anammox activity; Anammox.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100453
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Evaluation of microorganisms with sulfidogenic metabolic potential under anaerobic conditions BABT
Sakamoto,Isabel Kimiko; Maintinguer,Sandra Imaculada; Hirasawa,Julia Sumiko; Adorno,Maria Angela Tallarico; Varesche,Maria Bernadete Amâncio.
The aim of this work was to identify groups of microorganisms that are capable of degrading organic matter utilizing sulfate as an electron acceptor. The assay applied for this purpose consisted of running batch reactors and monitoring lactate consumption, sulfate reduction and sulfide production. A portion of the lactate added to the batch reactors was consumed, and the remainder was converted into acetic, propionic and butyric acid after 111 hours of operation These results indicate the presence of sulfate-reducing bacteria (SRB) catalyzing both complete and incomplete oxidation of organic substrates. The sulfate removal efficiency was 49.5% after 1335 hours of operation under an initial sulfate concentration of 1123 mg/L. The SRB concentrations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sulfate-reducing bacteria; Lactate; Most Probable Number; Organic acids.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500018
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