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Marinho,Anne C.T.A.; Vasconcelos,Santelmo; Vasconcelos,Emanuelle V.; Marques,Daniela A.; Benko-Iseppon,Ana Maria; Brasileiro-Vidal,Ana Christina. |
Abstract Jatropha is an important genus of Euphorbiaceae, with species largely used for various purposes, including the manufacturing of soaps and pharmaceutical products and applications in the bioenergetic industry. Although there have been several studies focusing J. curcas in various aspects, the karyotype features of Jatropha species are poorly known. Therefore, we analyzed six Jatropha species through fluorochrome staining (CMA/DAPI), fluorescent in situ hybridization (FISH) with 5S and 45S rDNA probes and genome size estimation by flow cytometry. Our results revealed several chromosome markers by both CMA/DAPI and FISH for the analyzed species. Five Jatropha species (J. curcas, J. gossypiifolia, J. integerrima, J. multifida and J. podagrica) showed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotaxonomy; DNA C-value; Heterochromatin; Physic nut; RDNA. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300442 |
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Bernardes,Ebenézer C.S.; Benko-Iseppon,Ana M.; Vasconcelos,Santelmo; Carvalho,Reginaldo; Brasileiro-Vidal,Ana C.. |
Endive (Cichorium endivia L.) and chicory (C. intybus L.) both have 2n = 18, but until now, there has been no detailed karyomorphological characterization. The present work evaluated five accessions of each species using FISH with rDNA probes and fluorochrome staining with CMA and DAPI. Both species presented distinct banding patterns after fluorochrome staining: while endive had proximal CMA++/DAPI- bands in the short arms of pairs 1, 2 and 3, chicory had proximal CMA-positive bands in chromosomes 1 and 3 and interstitial in the short arm of chromosome 8. Among endive accessions, FISH procedures revealed conserved position and number of 5S and 45S rDNA sites (two and three pairs, respectively), associated with the CMA-positive bands. Notwithstanding,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Compositae; Cichorieae; CMA/DAPI; RDNA sites; Chromosome rearrangements. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300010 |
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Vasconcelos,Emanuelle V.; Brasileiro-Vidal,Ana C.; Benko-Iseppon,Ana M.; Vasconcelos,Santelmo. |
ABSTRACT Aiming for a better understanding of karyotype evolution within Philodendron, we report chromosome counts for 23 species of the genus, of which 19 are being reported for the first time, thus increasing to 84 ( ca. 17 % of the genus) the total number of species with available chromosome counts. The diploid numbers 2 n = 32 and 2 n = 34 were the most common, with 10 and 11 species, respectively, whereas only two species presented different chromosome numbers ( P. giganteum with 2 n = 30 and P. adamantinum with 2 n = 36). The results are discussed in the context of previous analyses of karyotypes of Philodendron spp., taking into account bidirectional dysploidy as the main mechanism of chromosome number evolution within the genus. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aroids; Diploid number; Dysploidy; Karyotype; Philodendron. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000200309 |
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Cruz,Geyner Alves dos Santos; Vasconcelos,Santelmo; Pinangé,Diego Sotero de Barros; Gonçalves-Oliveira,Rodrigo Cesar; Alves,Marccus Vinícius; Torres,Rodrigo Augusto; Benko-Iseppon,Ana Maria. |
ABSTRACT The taxonomy of Cyperaceae is complex, with genera like Cyperus harboring species complexes. We analyzed the genetic similarity between Cyperus ligularis L. and C. odoratus L. based on DNA fingerprinting and cytogenetics. Significative genetic differentiation (G ST = 0.363) and low gene flow (N m = 0.877) indicated a clear genetic distinction between the two species. Moreover, the clustering analysis showed two distinct genetic groups, suggesting a lack of evidence for hybridization. The phenogram revealed two different lineages, and although all individuals of C. odoratus were collected from plots close to each other, they possessed greater genetic diversity than that observed among individuals of C. ligularis, which were sampled over a wider... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agmatoploidy; DNA amplification fingerprinting; Gene flow; Genetic differentiation; Hybridization. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000200264 |
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