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| Sereno,Fabiana Tavares Pires de Souza; Sereno,José Robson Bezerra; Vega-Pla,Jose Luis; Delgado,Juan Vicente. |
| We investigated the genealogy of the in situ conservation nucleus of the Pantaneiro horse using DNA microsatellites by evaluating 101 horses, the group consisting of 71 adult horses (3 stallions, 40 male and 31 mares) and 27 foals (14 colts and 13 fillies). Genomic DNA was extracted from hair roots and genotyped using 12 microsatellite markers (AHT4, AHT5, ASB2, ASB17, ASB23, HMS3 HMS6, HMS7, HTG4, HTG10, LEX33 and VHL20). The number of alleles per locus varied from 6 to 13, with a mean of 7.8 and the expected heterozygosity ranged from 0.544 to 0.734 (mean 0.644). The VLH20, ASB2, HTG10, ASB23 markers had a high (> 0.8) polymorphism information content and the total exclusion probability of the 12 microsatellite loci was 0.99. The genealogical study of... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Pantaneiro horse; Animal conservation; Microsatellites; Markers; Parentage verification. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100013 |
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| Sereno,Fabiana Tavares Pires de Souza; Sereno,José Robson Bezerra; Vega-Pla,Jose Luis; Kelly,Lucy; Bermejo,Juan Vicente Delgado. |
| The objective of this work was to evaluate the genetic diversity of Brazilian Pantaneiro horse by microsatellite markers, investigate the effect of genetic bottlenecks and estimate genetic differentiation among four horse breeds. Genetic variation was estimated through allele frequencies and mean breed heterozygosity. Nei's genetic distances among the breeds Pantaneiro, Thoroughbred, Arabian, Spanish Pure Breed (Andalusian), and Uruguay Creole were calculated, and it was used to construct an UPGMA dendrogram. Clustering at different K values was calculated to infer population structure and assign individuals to populations. Nei's distances showed a minimum distance between Pantaneiro horse and Spanish Pure Breed (0.228), and similar distances from Spanish... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equus caballus; Animal conservation; Molecular markers; Microsatellites; Genetic resources conservation. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000500007 |
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