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	Provedor de dados Braz. J. Plant Physiol. (1) Scientia Agricola (1) Autor Azevedo,Ricardo Antunes (2) Ferreira,Renato Rodrigues (2) Lea,Peter John (2) Vendemiatti,Ariane (2) Gratão,Priscila Lupino (1) Meinhardt,Lyndel Wayne (1) Mais... Palavra-chave Lysine (2) Threonine (2) Amino acids (1) Aspartate kinase (1) Aspartate pathway (1) Homoserine dehydrogenase (1) Maize (1) Methionine (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/report (1) Ano 2005 (1) 2004 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Isolation of enzymes involved in threonine biosynthesis from sorghum seeds Braz. J. Plant Physiol. Ferreira,Renato Rodrigues; Vendemiatti,Ariane; Meinhardt,Lyndel Wayne; Lea,Peter John; Azevedo,Ricardo Antunes. Cereal seeds are poor in essential amino acids, particularly lysine, tryptophan and threonine. The amino acids lysine and threonine are synthesized in the aspartate pathway. Although most of the enzymes of the aspartate pathway have been isolated and characterized in higher plant species, the metabolism of lysine and threonine is totally unknown in sorghum. We have isolated two enzymes, aspartate kinase (AK) and homoserine dehydrogenase (HSDH) from sorghum. Optimum assay conditions were established for the determination of AK and HSDH activities. The highest level of activity was observed in immature seeds. AK was shown to be inhibited by threonine and lysine indicating the existence of at least two isoenzymes, one sensitive to threonine inhibition and the... Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Aspartate kinase; Homoserine dehydrogenase; Lysine; Threonine. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202004000200005 Determination of aspartate kinase activity in maize tissues Scientia Agricola Ferreira,Renato Rodrigues; Vendemiatti,Ariane; Gratão,Priscila Lupino; Lea,Peter John; Azevedo,Ricardo Antunes. Lysine, threonine, methionine and isoleucine are synthesized from aspartate in a branched pathway in higher plants. Aspartate kinase plays a key role in the control of the aspartate pathway. The enzyme is very sensitive to manipulation and storage and the hydroxamate assay normally used to determine aspartate kinase activity has to be altered according to the plant species and tissue to be analyzed. We have optimized the assay for the determination of aspartate kinase in maize plants callus cell cultures. Among all the assay parameters tested, the concentration of ATP/Mg and temperature were critical for enzyme activity. In the case of temperature, 35°C was shown to be the optimum temperature for aspartate kinase activity. Tipo: Info:eu-repo/semantics/report Palavras-chave: Aspartate pathway; Maize; Lysine; Threonine; Methionine. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000200015 Registros recuperados: 2 Primeira ... 1 ... Última

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