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AFLP analysis of Xanthomonas axonopodis and X. arboricola strains used in xanthan production studies reveal high levels of polymorphism BJM
Mayer,Laurí; Silva,Wladimir Padilha da; Moura,Andrea Bittencourt; Vendruscolo,Claire Tondo.
Amplified fragment length polymorphism (AFLP) was used to analyze the genetic diversity of 14 strains of Xanthomonas arboricola pv. pruni and seven strains of X. axonopodis pv. phaseoli, which are used in xanthan production studies. Relationships identified by the AFLP profiles were assessed for xanthan production capacity, geographical location and host plant. Strains were isolated from 10 different geographic regions in South and Southeast States in Brazil. Data were analyzed for genetic similarity using the Dice coefficient and subjected to UPGMA cluster analysis. A total of 128 AFLP fragments were generated from four primer combinations: EcoRI+C/MseI+0, EcoRI+A/MseI+0, EcoRI+G/MseI+T and EcoRI+G/MseI+A. Of these, 96.1% were polymorphic. X. axonopodis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA fingerprints; Genomic diversity; Pathovar phaseoli; Pathovar pruni.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300026
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Structure of xanthan gum and cell ultrastructure at different times of alkali stress BJM
Luvielmo,Márcia de Mello; Borges,Caroline Dellinghausen; Toyama,Daniela de Oliveira; Vendruscolo,Claire Tondo; Scamparini,Adilma Regina Pippa.
Abstract The effect of alkali stress on the yield, viscosity, gum structure, and cell ultrastructure of xanthan gum was evaluated at the end of fermentation process of xanthan production by Xanthomonas campestris pv. manihotis 280-95. Although greater xanthan production was observed after a 24 h-alkali stress process, a lower viscosity was observed when compared to the alkali stress-free gum, regardless of the alkali stress time. However, this outcome is not conclusive as further studies on gum purification are required to remove excess sodium, verify the efficiency loss and the consequent increase in the polymer viscosity. Alkali stress altered the structure of xanthan gum from a polygon-like shape to a star-like form. At the end of the fermentation,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xanthomonas campestris; Xanthan production; Alkali stress; Transmission microscopy; Scanning electron microscopy.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100102
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