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	Provedor de dados BABT (1) BJM (1) Autor Vialta,Airton (2) Azevedo,João L (1) B. Junior,Renato (1) Campos,Fabíola R. (1) Catani,Cleide F (1) Dias,Ricardo Augusto (1) Genovez,Margareth E. (1) Keid,Lara Borges (1) Miyashiro,Simone (1) Piatti,Rosa M. (1) Scarcelli,Eliana (1) Mais... Palavra-chave Acremonium chrysogenum (1) B19 (1) Brucella abortus (1) Cephalosporin C (1) Cheese (1) PCR (1) Selenate resistant mutant (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2007 (1) 1999 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Isolation and characterization of selenate resistant mutants of Acremonium chrysogenum BABT Vialta,Airton; Catani,Cleide F; B. Junior,Renato; Azevedo,João L. Mutants unable to convert exogenous sulfate to sulfite were isolated using the toxic analogue selenate. Three of twenty-eight isolated mutants were chromate sensitive. They showed a possible lesion in the gene that codes the ATP sulfurylase. The others were chromate resistant, and probably had a lesion in one or both of the genes that code the sulfate permease. Methionine increased the resistance levels to selenate. In addition, the frequency of spontaneous mutants obtained in a medium containing methionine was higher (between 2.4 x 10-6 and 18.0 x 10-6) than that obtained using a medium without any intentional source of sulfur (between 0.7 x 10-6 and 5.0 x 10-6). The original strain, as well as the mutants, were able to grow in a sulfur-free liquid medium... Tipo: Info:eu-repo/semantics/article Palavras-chave: Selenate resistant mutant; Acremonium chrysogenum; Cephalosporin C. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131999000300016 Detection of Brucella abortus DNA in illegal cheese from São Paulo and Minas Gerais and differentiation of B19 vaccinal strain by means of the polymerase chain reaction (PCR) BJM Miyashiro,Simone; Scarcelli,Eliana; Piatti,Rosa M.; Campos,Fabíola R.; Vialta,Airton; Keid,Lara Borges; Dias,Ricardo Augusto; Genovez,Margareth E.. A total of 192 samples of illegal cheese from different regions of the states of São Paulo and Minas Gerais, Brazil, were analyzed for the isolation and detection of Brucella spp. DNA by means of microbiological culture and polymerase chain reaction (PCR), respectively. Samples that yielded positive results were submitted to the analysis of the occurrence of Brucella abortus (biovars 1, 2 e 4), as well as to the differentiation of DNA in B19 vaccinal strain or Brucella abortus field strain using PCR. Although the microorganism was not isolated from any sample, PCR detected 37 positive samples (19.27%) using genus-specific primers. From these, all (100%) were Brucella abortus. Differentiation of the strain showed that 30/37 samples (81.08%) were vaccinal... Tipo: Info:eu-repo/semantics/article Palavras-chave: Brucella abortus; B19; PCR; Cheese. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100005 Registros recuperados: 2 Primeira ... 1 ... Última

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