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| Mäder, P.; Vierheilig, H.; Streitwolf-Engel, R.; Boller, T.; Freyer, B.; Christie, P.; Wiemken, A.. |
| We studied the transport of "&N from a soil compartment separated from a plant root compartment by a hydrophobic polytetrafuoroethylene (PTFE) membrane to plants in the presence and absence of arbuscular mycorrhizal fungi (AMF). We have previously shown that this type of membrane efficiently inhibits mass fow and diffusion of mobile ions in the soil solution in an abiotic system, but can be penetrated by the hyphae of mycorrhizal fungi. Mycorrhizal tomatoes (Lycopersicon esculentum) colonized by Glomus mosseae were grown at two N fertilizer concentrations in a root compartment. A PTFE membrane was placed between the root compartment and an adjoining soil compartment that was inaccessible to the roots but accessible to the AMF hyphae (hyphal... |
| Tipo: Journal paper |
Palavras-chave: Soil biology. |
| Ano: 2000 |
URL: http://orgprints.org/26770/1/Maeder_et_al_2000_NP.pdf |
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| Vierheilig, H.; Alt-Hug, M.; Engel-Streitwolf, R.; Mäder, P.; Wiemken, A.. |
| The effects of tomato and bean rhizospheres on hyphal spreading of the arbuscular mycorrhizal (AM) fungus Glomus mosseae were studied using a soil compartment system in combination with hydrophobic polytetrafluorethylene (PTFE) membranes. Both the nylon screen and the PTFE membrane were freely permeable to hyphae but not to roots. Furthermore, the hydrophobic PTFE membrane seemed to be a barrier to the flux of soil solutions containing root exudates. The results show that water soluble exudates of tomato and bean roots greatly stimulate hyphal growth in the soil compartment system used. Moreover, water soluble root exudates of bean exert a clear attractional effect on AM hyphal growth. |
| Tipo: Journal paper |
Palavras-chave: Soil biology. |
| Ano: 1998 |
URL: http://orgprints.org/26774/1/Vierheilig_et_al_1998_PS_1.pdf |
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| Mäder, P.; Vierheilig, H.; Alt, M.; Wiemken, A.. |
| Various container systems have been described in which soil regions available to hyphae only are separated from the mycorrhizal root region by 30–60 μm mesh screens to study nutrient exchange between plants and fungi in the mycorrhizal symbiosis. The screens designed up to now prevent penetration by roots but allow easy passage of fungal hyphae as well as diffusion or mass flow of water and nutrient solutions. We tested hydrophobic microporous polytetrafluorethylene (PTFE) membranes (GORE-TEXR) with 5 to 15 μm diameter pores in an attempt to obtain a better seal between compartments and to prevent uncontrolled nutrient transport by diffusion or mass flow. We found that these membranes completely prevented diffusion or mass flow of ions between two soil... |
| Tipo: Journal paper |
Palavras-chave: Soil biology. |
| Ano: 1993 |
URL: http://orgprints.org/26785/1/Maeder_et_al_1993_PS.pdf |
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