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Sotres-Vega,A.; Baltazares-Lipp,M.; Villalba-Caloca,J.; Gaxiola-Gaxiola,M.O.; Santibañez-Salgado,J.A.; Olmos-Zúñiga,J.R.; Jasso-Victoria,R.. |
Cryopreservation has an immunomodulating effect on tracheal tissue as a result of class II antigen depletion due to epithelium exfoliation. However, not all epithelium is detached. We evaluated the role of apoptosis in the remaining epithelium of 30 cryopreserved tracheal grafts. Caspase-3 immunoreactivity of tracheal epithelium was studied in canine tracheal segments cryopreserved with F12K medium, with or without subsequent storage in liquid nitrogen at -196°C for 15 days. Loss of structural integrity of tracheal mixed glands was observed in all cryopreserved tracheal segments. Caspase-3 immunoreactivity in tracheal mucosa and in mixed glands was significantly decreased, in contrast to the control group and to cryopreserved tracheal segments in which it... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tracheal cryopreservation; Epithelium; Caspase-3. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200006 |
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