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| Villalobos,Patricio; Chávez,María Isabel; Olguín,Yusser; Sánchez,Elizabeth; Valdés,Erika; Galindo,Raúl; Young,Manuel E. |
| The inadequate treatments given to the served waste water which are disposal to the rivers and sea coast are the major sources of faecal Microorganisms and enteric bacterial pathogens. They are among the most serious effects of water pollution bringing risks on public health. None of the current methods for detection of pathogens offer real-time on site solutions, are capable of delivering a simple visual detection signal, or can be easily instrumented as an indicator of the presence of a pathogen in water. The use of lipid vesicles incorporating Polydiacetylenes (PDAs) for the development of biosensors for “real-time” detection of pathogens has become an alternative, due to its potential for simple colorimetric response against harmful... |
| Tipo: Journal article |
Palavras-chave: Bacterial detection; Biosensors; Lipid vesicles; PDA. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100004 |
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| Villalobos,Patricio; Rojas,M. Verónica; Conejeros,Pablo; Marshall,Sergio H.. |
| Conditions have been optimised to transfect the fish cell line CHSE-214 to measure expression, maintenance and putative chromosomal integration of the reporter gene LUC, spliced into two versions of an expression vector. The first is pCMVL, and the second p103, a novel pCMVL-derived plasmid to which a highly conserved tandem repeat from the salmon genome was added in an inverted configuration flanking the LUC gene to promote its chromosomal integration. A minimal ratio of one to one, lipopolyamine carrier to plasmid DNA, was enough to efficiently transfect the cell line to follow the fate of target DNAs up to five cell passages. In this time-span we demonstrated the maintenance of the foreign DNA in the cells, the concomitant expression of the reporter... |
| Tipo: Journal article |
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| Ano: 1999 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581999000200005 |
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| Enrione,Javier; Osorio,Fernando; López,Daniel; Weinstein-Oppenheimer,Caroline; Fuentes,Miguel A; Ceriani,Ricardo; Brown,Donald I; Albornoz,Fernando; Sánchez,Elizabeth; Villalobos,Patricio; Somoza,Rodrigo A; Young,Manuel E; Acevedo,Cristian A. |
| Gelatin, chitosan and hyaluronic acid are natural components used to prepare polymeric scaffold in tissue engineering. The physical properties of these materials confer an appropriate microenvironment for cells, which can be used as a regeneration system for skin and cartilage. In this work, we prepared and characterized a Gelatin/Chitosan/Hyaluronan lyophilized-polymer. Physical properties of lyophilized-polymer changed slightly with moisture, but when polymer was totally hydrated the elasticity changed significantly. Thermophysical characterisation indicated that temperatures higher than 30ºC could modify irreversibly the polymeric matrix probably due to protein denaturation. Besides, we used the polymer as scaffold to prepare a biosynthetic-skin,... |
| Tipo: Journal article |
Palavras-chave: Chitosan; Fibrin; Gelatin; Hyaluronic acid; Scaffold. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500020 |
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| Hernández,Marcela; Morgante,Verónica; Flores,Cecilia; Villalobos,Patricio; González,Myriam; Miralles,Pola; Dinamarca,Alejandro; Seeger,Michael. |
| The extensive use of s-triazine herbicides in diverse countries causes environmental and health concern. Simazine and atrazine are s-triazines widely used in agriculture and forestry. Although, natural dissipation of s-triazines in soils by physicochemical processes has been described, the main mechanism for their removal is biological degradation by microorganisms. Bioremediation is a successful strategy for the removal of i-triazines in soil. For bioaugmentation processes, s-triazine-degrading bacteria are required, which isolation from agricultural soils was described in this report. Studies of s-triazine adsorption and leaching in soil are useful to determine the bioavailability of these herbicides. The detection of s-triazine-degrading catabolic... |
| Tipo: Journal article |
Palavras-chave: Í-triazine; Simazine; Biodegradation; Bioremediation; Herbicide. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000200004 |
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