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| Certad, Gabriela; Zahedi, Alireza; Gantois, Nausicaa; Sawant, Manasi; Creusy, Colette; Duval, Erika; Benamrouz-vanneste, Sadia; Ryan, Una; Viscogliosi, Eric. |
| Current knowledge of Cryptosporidium species/genotypes in marine fish is limited. Following phylogenetic analysis at the 18S rDNA locus, a recent study identified six new genotypes of Cryptosporidium colonizing edible fish found in European seas. Of these, five grouped in a clade together (#Cryptofish 1–5) and one grouped separately (#Cryptofish 7). In the present study, after phylogenetic analyses of #Cryptofish1, #Cryptofish2, #Cryptofish4, #Cryptofish5 and #Cryptofish7 at the actin locus, the presence of two major clades was confirmed. In addition, when possible, longer 18S amplicons were generated. In conclusion, the small genetic distances between these genotypes designated as a novel marine genotype I (#Cryptofish 1-5) suggest that they may be... |
| Tipo: Text |
Palavras-chave: Piscine Cryptosporidium; Edible marine fish; Genetic characterization; 18S rDNA gene; Actin gene; Molecular phylogeny. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00665/77741/79864.pdf |
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| Certad, Gabriela; Follet, Jérôme; Gantois, Nausicaa; Hammouma-ghelboun, Ourida; Guyot, Karine; Benamrouz-vanneste, Sadia; Fréalle, Emilie; Seesao, Yuwalee; Delaire, Baptiste; Creusy, Colette; Even, Gaël; Verrez-bagnis, Veronique; Ryan, Una; Gay, Mélanie; Aliouat-denis, Cécile; Viscogliosi, Eric. |
| Cryptosporidium, a zoonotic pathogen, is able to infect a wide range of hosts including wild and domestic animals, and humans. Although it is well known that some parasites are both fish pathogens and recognized agents of zoonosis with a public health impact, little information is available concerning the prevalence of Cryptosporidium in wild aquatic environments. To evaluate the prevalence of Cryptosporidium spp. in commercially important edible marine fish in different European seas (English channel, North sea, Bay of Biscay, Celtic sea and Mediterranean sea), 1,853 specimens were collected as part of two surveys. Nested PCR followed by sequence analysis at the 18S rRNA gene locus was used to identify Cryptosporidium spp. The overall prevalence of... |
| Tipo: Text |
Palavras-chave: Cryptosporidium; Edible marine fish; 18S rRNA gene; Gp60; Molecular epidemiology; Phylogeny; Novel genotypes; European seas. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00497/60864/64198.pdf |
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| Monchy, Sebastien; Grattepanche, Jean-david; Breton, Elsa; Meloni, Dionigia; Sanciu, Giovanna; Chabe, Magali; Delhaes, Laurence; Viscogliosi, Eric; Sime-ngando, Telesphore; Christaki, Urania. |
| Background: Massive phytoplankton blooms, like the recurrent Phaeocystis proliferation observed every year in the Eastern English Channel (EEC), have a significant influence on the overall planktonic community structure and their food web dynamics. As well as being an important area for local fisheries, the EEC is an ideal ecosystem for work on microbial diversity. This is because, although its environmental context is relatively complex, it is reasonably well understood due to several years of monitoring and morphological observations of its planktonic organisms. The objective of our study was to better understand the under-explored microbial eukaryotic diversity relative to the Phaeocystis bloom. Methodology and Principal Findings: The community... |
| Tipo: Text |
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| Ano: 2012 |
URL: https://archimer.ifremer.fr/doc/00373/48460/48724.pdf |
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| Genitsaris, Savvas; Monchy, Sebastien; Viscogliosi, Eric; Sime-ngando, Telesphore; Ferreira, Stephanie; Christaki, Urania. |
| Previous microscopy-based studies in the eastern English Channel have revealed it to be a productive meso-eutrophic coastal ecosystem, characterized by strong repeating patterns in microplankton succession. The present study examines the seasonal structure of the entire protistan community from March 2011 to July 2013, using tag pyrosequencing of the V2-V3 hypervariable region of the 18S rRNA gene. A total of 1242 OTUs and 28 high-level taxonomic groups, which included previously undetected taxa in the area, were identified. The detected OTUs were considered according to taxon-specific traits, which included their trophic role, abundance and specialization level. Taxa differentiation based on specialization level rather than abundance was more informative... |
| Tipo: Text |
Palavras-chave: Unicellular eukaryotes; Tag pyrosequencing; 18S rRNA gene; Succession; Generalists; Specialists. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00373/48454/48738.pdf |
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