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	Provedor de dados Electron. J. Biotechnol. (2) Autor Lavigne,Rob (2) Volckaert,Guido (2) Chibeu,Andrew (1) Cornelis,Pierre (1) Mattheus,Wesley (1) Matthijs,Sandra (1) Mais... Palavra-chave Cognate gene plasmid (1) Cosmid (1) Functional complementation homolog Saccharomyces cerevisiae yeast (1) PRG930cm (1) Pseudomonas (1) Sequencing (1) Mais... Tipo do documento Journal article (2) Ano 2009 (1) 2007 (1) País Chile (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Functional complementation in Saccharomyces cerevisiae under control of the natural yeast promoter Electron. J. Biotechnol. Lavigne,Rob; Volckaert,Guido. A novel approach for functional complementation of foreign genes in Saccharomyces cerevisiae is presented. This approach is based on the use of the widely available cognate gene plasmids (e.g. pRS416) of the European Functional Analysis Network (EUROFAN). The functional complementation of the human homolog of YOR159c (SME1 gene) shown here is the first demonstration of complementation using the original yeast promoter, theoretically offering a more natural regulation of protein expression Tipo: Journal article Palavras-chave: Cognate gene plasmid; Functional complementation homolog Saccharomyces cerevisiae yeast. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000200014 Genetic and physical map of broad host range cosmid pRG930cm Electron. J. Biotechnol. Chibeu,Andrew; Lavigne,Rob; Mattheus,Wesley; Matthijs,Sandra; Cornelis,Pierre; Volckaert,Guido. We hereby present the complete sequence and annotation of pRG930cm, a spectinomycin/streptomycin/chloramphenicol-resistant cosmid vector. pRG930cm (17,256 bp; GenBank Accession No.: FM174471) has a broad host range, and is stably maintained by a number of Gram-negative bacteria including Pseudomonas spp, Escherichia coli, Agrobacterium tumefaciens and Azorhizobium caulinodans ORS571. pRG930cm is already widely used and its sequence will aid efficient construction and analysis of cosmid libraries. Tipo: Journal article Palavras-chave: Cosmid; PRG930cm; Pseudomonas; Sequencing. Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200010 Registros recuperados: 2 Primeira ... 1 ... Última

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