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Isolation, identification and characterization of regional indigenous Saccharomyces cerevisiae strains BJM
Šuranská,Hana; Vránová,Dana; Omelková,Jiřina.
Abstract In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts. The grape berries (Sauvignon blanc and Pinot noir) were grown under the integrated and organic mode of farming in the South Moravia (Czech Republic) wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typing were employed to differentiate among indigenous S. cerevisiae strains. This combination of the methods provides a rapid and relatively simple approach for identification of yeast of S. cerevisiae at strain level. In total, 120 isolates were identified and grouped...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces genus; Oenological properties; ITS-PCR-RFLP; Interdelta PCR typing; Species-specific primers.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100181
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