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| BHUIYAN, Mohammad Musharraf Uddin; SUZUKI, Yo; WATANABE, Hiroyuki; LEE, Eunsong; HIRAYAMA, Hiroki; MATSUOKA, Koji; FUJISE, Yoshihiro; ISHIKAWA, Hajime; OHSUMI, Seiji; FUKUI, Yutaka; 福井, 豊. |
| The objectives of this study were to choose an effective embryo reconstruction method and an effective post-activation agent for in vitro production of sei whale (Balaenoptera borealis) interspecies somatic cell nuclear transfer (iSCNT) embryos. Moreover, trichostatin A (TSA) treatment of whale iSCNT embryos was performed to improve the in vitro embryo development. In Experiment 1, the fusion rate was significantly higher (88.1%) in embryos reconstructed using the intracytoplasmic cell injection method (ICI) than that (48.7%) in the subzonal cell insertion (SUZI) counterpart. The rates of pseudopronucleus (PPN) formation (77.4 vs. 77.2%) and cleavage (24.5 vs. 37.0%) did not vary between ICI and SUZI. However, the PPN formation and cleavage rates were... |
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Palavras-chave: Interspecies; Somatic cell nuclear transfer; Whale. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2815 |
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| SUZUKI, Yo; WATANABE, Hiroyuki; FUKUI, Yutaka; 福井, 豊. |
| The aim of this study was to investigate whether seasonal changes affected in vitro developmental competence of porcine oocytes. The relationship between atmospheric temperature and embryonic development of in vitro matured porcine oocytes following intracytoplasmic sperm injection was examined throughout the year. The blastocyst rate (31.1%) in winter (mean atmospheric temperature during December to February: -3.8 C) was significantly higher (P<0.05) than those of other seasons in 2008/2009 (19.7-23.5%; 6.3-17.5 C). The monthly mean blastocyst rates were negatively correlated with the temperatures (r=-0.5944, P<0.05). The results of the present study suggest that porcine embryos could be produced throughout the year, but the in vitro production... |
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Palavras-chave: Embryonic development; Pig; Seasonal changes; Temperature. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2814 |
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| FUKUI, Yutaka; KOHNO, Hirohide; OKABE, Kentaro; KATSUKI, Sara; YOSHIZAWA, Masahiro; TOGARI, Tetsuro; WATANABE, Hiroyuki; 福井, 豊. |
| In this study, two successive field trials were conducted during the non-breeding season to investigate various factors affecting on fertility of Suffolk ewes after intrauterine insemination with frozen-thawed semen. In the first year (Experiment 1), three sperm numbers per insemination dose (0.25, 0.5 and 1 million sperm) and five sheep farms were used, and in the second year (Experiment 2), parity, age, body weight, body condition score (BCS) and postpartum days were investigated to compare pregnancy and lambing rates. High pregnancy and lambing rates (70.6 and 70.6%, respectively) were obtained with 0.25 million sperm per dose. There were no significant differences in the pregnancy and lambing rates among the five farms, but there was a tendency for one... |
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Palavras-chave: Artificial insemination; Fertility; Frozen semen; Sheep. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2817 |
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