|
Van Beek, E.; Criel, G.R.J.; Walgraeve, H.; De Loof, A.. |
The <i>Artemia</i> strain from Great Salt Lake (GSL 375) was used. Total body extracts and hemolymph of <i>Artemia</i> were purified by solvent partition and column chromatography. Moulting-hormone concentrations were then determined by a specific radioimmunoassay (RIA). Results are expressed in ng ecdysteroid activity/g fresh weight (or per ml). Initially, the RIA data on extracts of adults were highly variable. The use of a more accurate sampling technique based on the reproductive cycle of the females allowed to determine a hormone activity pattern.In females, moulting cycles alternate with vitellogenic cycles. The lowest values of ecdysone (3-6 ng ecdysone equivalents/g fresh body weight or ml hemolymph) occur during... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hormones; Moulting; Artemia. |
Ano: 1987 |
URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3209 |