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Physicochemical and Biological Investigation of Different Structures of Carbon Coatings Deposited onto Polyurethane BABT
Kaczorowski,Witold; Batory,Damian; Jakubowski,Witold; Szymanski,Witold; Komorowski,Piotr; Walkowiak,Bogdan; Sanak,Marek; Niedzielski,Piotr.
The aim of this study was to examine the thrombogenic properties of polyurethane that was surface modified with carbon coatings. Physicochemical properties of manufactured coatings were investigated using transmission electron microscopy (TEM), atomic force microscopy (AFM), X-ray Photoelectron Spectroscopy (XPS), Raman spectroscopy and contact angle measurement methods. Samples were examined by the Impact-R method evaluating the level of platelets activation and adhesion of particular blood cell elements. The analysis of antimicrobial resistance against E. coli colonization and viability of endothelial cells showed that polyurethane modified with use of carbon layers constituted an interesting solution for biomedical application.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diamond-like carbon; Atomic force microscopy (AFM); X-ray photoelectron spectroscopy XPS; Raman spectroscopy; Biological properties.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100600
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Resistance of oxidative stress in biofilm and planktonic cells BABT
Jakubowski,Witold; Walkowiak,Bogdan.
This work studied the susceptibility of biofilm produced by E. coli to oxidative stress, and compared the components of free radicals defences: level of glutathione, catalase and dismutase activities in planktonic and biofilm located cells. Results showed the diversity of responses to oxidative stress in bacterial cells in log or stationary phases in both planktonic and biofilm forms. The bacteria were exposed to free-radical donors (H2O2, tBOOH, menadione, SIN-1 or peroxynitrite) in a wide range of final concentrations, from 0.5 to 10mM. Different level of toxicity of individual donors, independence of cell type (planktonic forms or biofilm) and phases of growth were observed. The highest oxidative stress resistance was observed for the cells in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Reactive oxygen species; Biofilm; Bacterial colonization; Planktonic cells; Implant.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200300
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