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	Provedor de dados BABT (2) Autor Ramalho Neto,Eduardo (2) Wanderlei-Silva,Denise (2) Gonzaga,Rosa Silva (1) Hanlin,Richard (1) Nobre,Mariana (1) Viana,Luciana Silva (1) Mais... Palavra-chave PCR (2) 18S ribosomal DNA (1) DNA purification (1) DNA sequencing (1) Human papillomavirus (1) Phyllachorales (1) RFLP (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2005 (1) 2003 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Molecular systematics of the Phyllachorales (ascomycota, fungi) based on 18S ribosomal DNA sequences BABT Wanderlei-Silva,Denise; Ramalho Neto,Eduardo; Hanlin,Richard. In order to evaluate the monophyly of the Phyllachorales from a molecular standpoint and elucidate its phylogenetic relationships with other orders, a segment of the 18S rRNA gene from several representatives of the Phyllachorales, including species of Glomerella, Phyllachora, Coccodiella (=Coccostroma), Sphaerodothis, Ophiodothella, as well as Magnaporthe was sequenced. Maximum Parsimony analysis revealed that the Phyllachorales was a polyphyletic assemblage of taxa. None of the other members of the Phyllachorales, which produced either a clypeus or stroma, clustered with Glomerella. Of the taxa examined, was Coccodiella the closest relative of Phyllachora. Magnaporthe was closely related to the Diaporthales. Our 18S rDNA data highly supported Glomerella... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA sequencing; 18S ribosomal DNA; PCR; Phyllachorales. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300002 High quality DNA from human papillomavirus (HPV) for PCR/RFLPs BABT Wanderlei-Silva,Denise; Nobre,Mariana; Gonzaga,Rosa Silva; Viana,Luciana Silva; Ramalho Neto,Eduardo. The analysis of DNA in clinical samples for a secure diagnostic has become indispensable nowadays. Techniques approaching isolation of high molecular weigth DNA of HPV could lead to efficient amplification and early clinical diagnosis of the virus DNA by PCR (polymerase chain reaction). We describe a fast, non-toxical, efficient and cheap method for DNA isolation of human papilloma virus (HPV) from cervical smears using guanidine (DNAzol solution). A 450 bp DNA band correponding to the late region (L1) of the virus genome was detected by PCR, showing that the DNAzol extraction soluction generated a good viral DNA yield. The electrophoretic pattern after digestion with restriction endonucleases (RFLPs/PCR) revealed the predominance of HPV-16 and HPV-33 in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human papillomavirus; DNA purification; PCR; RFLP. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100006 Registros recuperados: 2 Primeira ... 1 ... Última

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