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| Bai, Chang-ming; Morga, Benjamin; Rosani, Umberto; Shi, Jie; Li, Chen; Xin, Lu-sheng; Wang, Chong-ming. |
| Ostreid herpesvirus 1 (OsHV-1) is an important pathogen associated with mass mortalities of cultivated marine mollusks worldwide. Since no cell line allows OsHV-1 replication in vitro, it is difficult to isolate enough high-purity viral DNA for High-Throughput Sequencing (HTS). We developed an efficient approach for the enrichment of OsHV-1 DNA for HTS with long-range PCR. Twenty-three primer pairs were designed to cover 99.3% of the reference genome, and their performances were examined on ten OsHV-1 infected samples. Amplicon mixtures from six successfully amplified samples were sequenced with Illumina platform, and one of them (ZK0118) was also sequenced with the PacBio platform. PacBio reads were assembled into 2 scaffolds compared to 9−68 scaffolds... |
| Tipo: Text |
Palavras-chave: Long-range PCR; OsHV-1; High-throughput sequencing; Evolution; Diversity. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00464/57566/64184.pdf |
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| Bai, Chang-ming; Wang, Qing-chen; Morga, Benjamin; Shi, Jie; Wang, Chong-ming. |
| We investigated the susceptibility of ark shell, Scapharca broughtonii, adults to Ostreid herpesvirus SB strain (OsHV-1-SB) through experimental infection by intramuscular injection assays. Results showed the onset of mortality occurred at 3 days post injection, one day after the water turbidity became evident in rearing tanks. The mortality curves for the challenged group were similar to those observed at affected hatcheries. Histological lesions, herpesvirus-like particles and high OsHV-1-SB quantities were detected in challenged ark shells. This is the first study to successfully reproduce OsHV-1 disease in Arcoida species, and very few studies in adult bivalves (over 24 months old). |
| Tipo: Text |
Palavras-chave: Scapharca broughtonii; Ostreid herpesvirus SB strain; OsHV-1-SB; Experimental infection; Viral quantity. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00360/47119/47081.pdf |
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