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| Zhao,Qing; Jing,Ji; Wang,Gang; Wang,Jie Hua; Feng,Yuan Yuan; Xing,Han Wen; Guan,Chun Feng. |
| Although Agrobacterium-mediated transformation protocols for many economically important plant species have been well established, protocol for a number of flowering plants including Anthurium andraeanum remains challenging. In this study, we report success in generating transgenic Anthurium andraeanum cv Arizona using Agrobacterium GV3101 strain harboring a binary vector carrying gfp as a reporter gene. The possibility of facilitating the screening process for transgenic plants expressing functional proteins using gfp marker was explored. In order to realize high transformation efficiency, different explant sources including undifferentiated callus pieces and petioles were compared for their regeneration efficiency and susceptibility to... |
| Tipo: Journal article |
Palavras-chave: Cocultivation; Gene transfer; Green fluorescent protein; Regeneration. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500009 |
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