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| Wang,Xiu-Fang; Jin,Xia; Wang,Xiaoyan; Liu,Jing; Feng,Jingjing; Yang,QinQing; Mu,Wenli; Shi,Xiaojuan; Lu,Zhanjun. |
| The retrotransposon known as long interspersed nuclear element-1 (L1) is 6 kb long, although most L1s in mammalian and other eukaryotic cells are truncated. L1 contains two open reading frames, ORF1 and ORF2, that code for an RNA-binding protein and a protein with endonuclease and reverse transcriptase activities, respectively. In this work, we examined the effects of full length L1-ORF2 and ORF2 fragments on green fluorescent protein gene (GFP) expression when inserted into the pEGFP-C1 vector downstream of GFP. All of the ORF2 fragments in sense orientation inhibited GFP expression more than when in antisense orientation, which suggests that small ORF2 fragments contribute to the distinct inhibitory effects of this ORF on gene expression. These results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; Green fluorescent protein gene; L1-ORF2; Transcription termination; Orientation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400003 |
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| Shao,Huanhuan; Yong,Bin; Xu,Pan; Zheng,Haiyan; Liao,Ruoxing; Wang,Xiaoyan; Li,Xinyu; Zhang,Li; Shen,Jiabin. |
| ABSTRACT Phytoene synthase (PSY) is the rate-limiting enzyme for carotenoid biosynthesis. To date, several studies focused on PSY genes in the context of abiotic stress responses. In this study, two phytoene synthase encoding genes, IbPSY1 and IbPSY2, were identified from a published transcriptome and bioinformatic analysis showed that they shared conserved domains with phytoene synthases from other plants. The IbPSY1 gene was cloned and carefully characterized. Digital gene expression profiling (DGE) showed that the highest transcription level of IbPSY1 was in young leaves, and the lowest level was in stems. In vivo expression levels of IbPSY1 under abiotic stress were observed to be highest in stems at day 11. Over-expression of IbPSY1 in Escherichia... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ipomoea batatas; Phytoene synthase; Abiotic stress. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100422 |
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