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	Provedor de dados OAK (1) Autor Akiyama, Junko (1) Bhuiyan, Mohammad Musharraf Uddin (1) Fukui, Yutaka (1) Watanabe, Hiroyuki (1) 福井, 豊 (1) Palavra-chave Assisted reproductive technology (1) Dithiothreitol (1) Intracytoplasmic sperm injection (1) Oocyte activation (1) Ano 2009 (1) País Japan (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Enhanced Oocyte Activation by Intracytoplasmic Injection of Porcine Spermatozoa Pre-treated with Dithiothreitol OAK Watanabe, Hiroyuki; Akiyama, Junko; Bhuiyan, Mohammad Musharraf Uddin; Fukui, Yutaka; 福井, 豊. The aim of this study was to confirm the effect of dithiothreitol (DTT)-treated spermatozoa on oocyte activation following intracytoplasmic sperm injection (ICSI). Boar spermatozoa with or without DTT treatment (5 mM, 30 min) were injected into in vitro matured porcine oocytes, and the nuclear phase in presumptive zygotes was observed at 3 h intervals up to 12 h after ICSI. Furthermore, developmental competence of embryos produced by DTT-treated or non-treated spermatozoa was monitored after cultivation in vitro for 144 h. Male and female pronuclear formation rates in the oocytes injected with DTT-treated spermatozoa were significantly (P < 0.05) higher than those in the oocytes injected with non-treated spermatozoa. Additionally, we observed that... Palavras-chave: Assisted reproductive technology; Dithiothreitol; Intracytoplasmic sperm injection; Oocyte activation. Ano: 2009 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2803 Registros recuperados: 1 Primeira ... 1 ... Última

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