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The characterization and composition of bacterial communities in soils blended with spent foundry sand NWISRL
Dungan, R.S.; Kim, J.; Weon, H.; Leytem, A.B..
The purpose of this research was to characterize the structure and composition of bacterial communities in sandy loam and silty clay soils amended with 30% spent sand from iron, aluminum, and steel foundries. All spent foundry sand (SFS) blends were grown with or without perennial ryegrass and samples were collected at 4 weeks and 6 months. Regions of the 16S rRNA gene were amplified using PCR and subsequently analyzed by DGGE and sequenced for bacterial identification and phylogenetic classification. Cluster analyses of PCR-DGGE banding patterns revealed that SFS blends from week 4 and month 6 produced unique clusters, with most ryegrass treatments clustering away from those without ryegrass. The diversity of the bacterial community revealed that it...
Tipo: Article Palavras-chave: Soil; Soil quality.
Ano: 2009 URL: http://eprints.nwisrl.ars.usda.gov/1339/1/1316.pdf
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