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Genome sequence and genetic linkage analysis of Shiitake mushroom _Lentinula edodes_ Nature Precedings
Hoi Shan Kwan; Chun Hang Au; Man Chun Wong; Jing Qin; Iris Sze Wai Kwok; Winnie Wing Yan Chum; Pui Ying Yip; Kin Sing Wong; Lei Li; Qian Li Huang; Wen Yan Nong.
_Lentinula edodes_ (Shiitake/Xianggu) is an important cultivated mushroom. Understanding the genomics and functional genomics of _L. edodes_ allows us to improve its cultivation and quality. Genome sequence is a key to develop molecular genetic markers for breeding and genetic manipulation. We sequenced the genome of _L. edodes_ monokaryon L54A using Roche 454 and ABI SOLiD genome sequencing. Sequencing reads of about 1400Mb were de novo assembled into a 40.2 Mb genome sequence. We compiled the genome sequence into a searchable database with which we have been annotating the genes and analyzing the metabolic pathways. In addition, we have been using many molecular techniques to analyze genes differentially expressed during development. Gene ortholog groups...
Tipo: Poster Palavras-chave: Genetics & Genomics.
Ano: 2012 URL: http://precedings.nature.com/documents/6855/version/1
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