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Relative expression of mRNAs related to cavitation process in bovine embryos produced in vivo and in vitro R. Bras. Zootec.
Wohlres-Viana,Sabine; Boite,Mariana Cortes; Viana,João Henrique Moreira; Machado,Marco Antonio; Camargo,Luiz Sérgio de Almeida.
The objectives of this work were to identify and to evaluate possible differences on gene expression of aquaporins and Na/K-ATPases transcripts between embryos in vivo and in vitro produced. For each group, 15 blastocysts distributed in three pools were used for RNA extraction followed by amplification and reverse transcription. The resulting cDNAs were submitted to Real-Time PCR, using the GAPDH gene as endogenous control. It was not possible to identify AQP1 transcripts. Relative expression of AQP3 (1.33 ± 0.78) and AQP11 (2.00 ± 1.42) were not different in blastocysts in vitro and in vivo produced. Na/K-ATPase α1 gene (2.25 ± 1.07) was overregulated whereas Na/K-ATPase β2 transcripts 0.40 ± 0.30) did not differ among blastocysts produced in vitro from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Blastocoel; Gene expression; Osmotic gradient; Water channel.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000100017
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