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	Provedor de dados Biol. Res. (1) Genet. Mol. Biol. (1) Autor Wu,Jie (2) Cai,Ping-Zhong (1) Chen,Pu (1) Chen,Xiangmei (1) Fang,Ji-Chao (1) Geng,Wenjia (1) Guo,Hui-Fang (1) Liu,Shuwen (1) Tang,Li (1) Wei,Ribao (1) Yan,Wen-Zhao (1) Yin,Zhong (1) Zhang,Xueguang (1) Zhong,Wan-Fang (1) Zhu,Fei (1) Zhu,Hanyu (1) Mais... Palavra-chave Bacillus thuringiensis serovar sotto (1) Characterization analysis (1) Chitinase (1) Chronic anti-Thy-1 glomerulonephritis (1) Erk (1) Gene cloning (1) Mesangial cell proliferation (1) Shenhua Tablet (1) Touchdown PCR (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2016 (1) 2005 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Cloning of the Bacillus thuringiensis serovar sotto chitinase (Schi) gene and characterization of its protein Genet. Mol. Biol. Zhong,Wan-Fang; Fang,Ji-Chao; Cai,Ping-Zhong; Yan,Wen-Zhao; Wu,Jie; Guo,Hui-Fang. Chitinase plays a positive role in the pathogenicity of Bacillus thuringiensis to insect pests. We used touchdown PCR to clone the chitinase (Schi) gene from Bacillus thuringiensis serovar sotto (Bt sotto) chromosomal DNA. Our DNA sequencing analysis revealed that the Bt sotto Schi gene consists of an open reading frame (ORF) of 2067 nucleotides with codes for the chitinase precursor. We also found that the putative promoter consensus sequences (the -35 and -10 regions) of the Bt soto Schi gene are identical to those of the chiA71 gene from Bt Pakistani, the chiA74 gene from Bt kenyae and the ichi gene from Bt israelensis. The Schi chitinase precursor is 688 amino acids long with an estimated molecular mass of 75.75 kDa and a theoretical isoelectric point... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus thuringiensis serovar sotto; Chitinase; Touchdown PCR; Gene cloning; Characterization analysis. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500026 Shenhua Tablet inhibits mesangial cell proliferation in rats with chronic anti-Thy-1 nephritis Biol. Res. Geng,Wenjia; Wei,Ribao; Liu,Shuwen; Tang,Li; Zhu,Hanyu; Chen,Pu; Wu,Jie; Zhang,Xueguang; Zhu,Fei; Yin,Zhong; Chen,Xiangmei. BACKGROUND: In China, mesangial proliferative glomerulonephritis (MsPGN) is one of the most common kidney diseases. In this study, we treated a rat model of chronic anti-Thy-1 MsPGN with Shenhua Tablet and evaluated whether the tablet was able to protect the kidney function. Thirty-six Wistar rats were randomly divided into six groups: (1) Sham surgery (Sham); (2) anti-Thy-1 nephritis model (Thy-1); (3) anti-Thy-1 nephritis model + irbesartan-treated (Irb); (4) anti-Thy-1 nephritis model + low-dose of Shenhua Tablet (SHL); (5) anti-Thy-1 nephritis model + medium-dose of Shenhua Tablet (SHM); (6) anti-Thy-1 nephritis model + high-dose of Shenhua Tablet (SHH RESULTS: Thirteen weeks after drug treatment, urinary proteins were quantified and renal pathological... Tipo: Journal article Palavras-chave: Shenhua Tablet; Chronic anti-Thy-1 glomerulonephritis; Mesangial cell proliferation; Erk. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100017 Registros recuperados: 2 Primeira ... 1 ... Última

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