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Baloch,Zulqarnain; Yasmeen,Nafeesa; Li,Yuanyue; Ma,Ke; Wu,Xiaomei; Yang,Shi-hua; Xia,Xueshan. |
ABSTRACT Background: Dai is a major Chinese ethnic minority group residing in rural areas of the southern part of Yunnan. However, no data exist on the Human Papillomavirus (HPV) prevalence and genotype distribution among Dai women. Method: A total of 793 participants (Dai = 324, Han = 251, other ethnic = 218) were included in this study. PCR was performed to detect the HPV-positive samples, and genotyping was performed with an HPV Geno-Array. Result: The overall HPV prevalence was very low among Dai women compared to the others. The prevalence of high-risk-HPV infections was significantly higher (p = 0.001) among other ethnic women (22.0%) than that among Han (13.1%) and Dai women (7.1%). The overall HPV, high-risk-HPV, single and multiple infection... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HPV; Prevalence; Dai; Genotypes; Rural; Urban; China. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000300325 |
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Li,Chao; Fu,Gongyu; Shi,Yaoqiang; Zhang,A-Mei; Xia,Xueshan; Fang,Yue; Mao,Xiaoqin; Jiang,Jie; Song,Yuzhu; Yang,Guangying. |
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish a loop-mediated isothermal amplification (LAMP) method for the rapid and sensitive detection of K. pneumoniae-specific gene ureR_1 (Gene ID: 11847803). The ureR_1 gene was obtained through local and online BLAST, and the specific primers were designed for its detection. Positive reactions were observed on all 140 K. pneumoniae clinical isolates while all the 82 non-K. pneumoniae clinical isolates were negative. Plasmids with the specific gene and the mouse... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Loop-mediated isothermal amplification; Polymerase chain reaction; Klebsiella pneumoniae; Novel specific gene; Specific; Sensitive method. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300609 |
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