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| Yang,Weichao; Han,Litao; Mandlaa,Mandlaa; Zhang,Haihong; Zhang,Zhongze; Xu,Hui. |
| Abstract A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94.45%) of l-sorbose to 2-KLG. In contrast to the traditional screening method, this one significantly improved the frequency of obtaining positive mutants. The proposed plate screening method is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ketogulonicigenium vulgare; PH indicator; Rapid screening; Spaceflight mutation; 2-Keto-l-gulonic acid. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300397 |
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| Li,Shi Lin; Lin,Qiang; Li,Xin Ran; Xu,Hui; Yang,Yun Xi; Qiao,Dai Rong; Cao,Yi. |
| Microbial lipids, which are also known as single cell oils (SCO), are produced by oleaginous microorganisms including oleaginous bacteria, yeast, fungus and algae through converting carbohydrates into lipids under certain conditions. Due to its unique environment having extremely low temperature and anoxia, the Tibetan Plateau is amongst the regions with numerous rare ecotypes such as arid desert, salt marsh, alpine permafrost, hot spring, and lawn. By using a rapid, convenient screening method, we identified 31 strains of oleaginous microorganisms from different habitats in the Tibetan Plateau, which include wetlands, lawn, hot spring, alpine permafrost, and saline-alkali soil. Molecular identity analysis showed that they belong to 15 different species, 7... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oleaginous microorganisms; Screening; The Tibetan Plateau; Biodiversity. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200026 |
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| Yi,Xiuli; Shi,Yan; Xu,Hui; Li,Wei; Xie,Jie; Yu,Rongqing; Zhu,Jun; Cao,Yi; Qiao,Dairong. |
| The analysis of individual gene product should enable to clarify the role of a particular enzyme in a complex xylanase system of A. niger. The two genes encoding precursors of co-produced endo-1,4-β-D-xylanases, xynA1 and xynB, were isolated from Aspergillus niger SCTCC 400264 (SCTCC, China) by using RT-PCR technique and then successfully expressed in Escherichia coli BL21. The nucleotide sequences of the xynA1 and xynB genes revealed that they were only 52.5% homology to each other. Characterization of the recombinant enzymes revealed the different properties: the specific activity of recombinant XYNA1 was 16.58 U/mg compared to 1201.7 U/mg for recombinant XYNB; The optimum temperature and pH of the recombinant XYNA1 were 35 ºC and 3.0, respectively,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus niger; Xylanase; Prokaryotic expression; Enzymatic characterization. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300030 |
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