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Adhesive properties of an outer structure of Clostridium perfringens type A isolated from piglets with with catarrhal enteritis Rev. Microbiol.
Teixeira,Elizabeth Pelosi; Serafim,Marlene Braide; Höfling,Maria Alice Cruz; Yamada,Aureo T.; Castro,Antonio Fernando Pestana de.
One strain (S32) of Clostridium perfringens type A was isolated from a case of catarrhal enteritis of piglets. This strain was able to adhere to HeLa cells showing an adherence index (AI) of 25.15 ± 1.26 (mean ± 1 standard error of the mean). Treatment of the bacterial cells with trypsin (0.25mg/ml) decreased in 70%-80% the AI and metaperiodate (10mg/ml) abolished completely the adherence, suggesting that the structure responsible for this phenomenon was probably a glycoprotein. Heating of bacterial suspensions (100ºC/5 min) before carrying out the adhesion test decreased the AI rendering it equal to the negative controls. Rabbit homologous S32 antiserum inhibited the adherence up to dilutions of 1: 640, at least. The piglet ileal loop assay, carried out...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cl. perfringens type A; Adherence; Fibrillar structure; Enteritis; Piglets.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000300010
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