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| Yamaguti,M.; Muller,E.E.; Piffer,A.I.; Kich,J.D.; Klein,C.S.; Kuchiishi,S.S.. |
| Since Mycoplasma hyopneumoniae isolation in appropriate media is a difficult task and impractical for daily routine diagnostics, Nested-PCR (N-PCR) techniques are currently used to improve the direct diagnostic sensitivity of Swine Enzootic Pneumonia. In a first experiment, this paper describes a N-PCR technique optimization based on three variables: different sampling sites, sample transport media, and DNA extraction methods, using eight pigs. Based on the optimization results, a second experiment was conducted for testing validity using 40 animals. In conclusion, the obtained results of the N-PCR optimization and validation allow us to recommend this test as a routine monitoring diagnostic method for Mycoplasma hyopneumoniae infection in swine herds. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Swine Enzootic Pneumonia; Mycoplasma hyopneumoniae; Diagnosis; Nested- PCR. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300011 |
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