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| Borges,R.M.; Horne,J.H.; Melo,A.; Vidal,J.T.; Vieceli,F.M.; Melo,M.O.; Kanno,T.Y.N.; Fraser,S.E.; Yan,C.Y.I.. |
| One of the challenges of the postgenomic era is characterizing the function and regulation of specific genes. For various reasons, the early chick embryo can easily be adopted as an in vivo assay of gene function and regulation. The embryos are robust, accessible, easily manipulated, and maintained in the laboratory. Genomic resources centered on vertebrate organisms increase daily. As a consequence of optimization of gene transfer protocols by electroporation, the chick embryo will probably become increasingly popular for reverse genetic analysis. The challenge of establishing chick embryonic electroporation might seem insurmountable to those who are unfamiliar with experimental embryological methods. To minimize the cost, time, and effort required to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Electroporation; In vivo gene expression; Electroporator; Vertebrate embryo. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000900752 |
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| Rossi,E.; Siwiec,F.; Yan,C.Y.I.. |
| The dorsoventral axis of the eye is determined prior to optic cup invagination. A variety of signaling pathways have been implicated in the maintenance of the optic dorsoventral axis, including, but not limited to, bone morphogenetic protein 4, Sonic Hedgehog and retinoic acid. Here, we investigated the possible contribution of Wnt ligands to the establishment or maintenance of the optic axis by analyzing their expression pattern during early chick optic development. We performed in situ hybridization of Wnt-1, Wnt-3a, Wnt-4, and Wnt-5a during the optic vesicle, early optic cup and established optic cup stages and focused our analysis on the optic region. Our data showed that Wnt-5a, but none of the others, is expressed in the dorsal region of the eye... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Wnt-5a; Optic vesicle; Optic development; Wnt; Anterior neural tube. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000005 |
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