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| Anbazhagan,Deepa; Mui,Wang Seok; Mansor,Marzida; Yan,Gracie Ong Siok; Yusof,Mohd Yasim; Sekaran,Shamala Devi. |
| Nosocomial infections are major clinical threats to hospitalised patients and represent an important source of morbidity and mortality. It is necessary to develop rapid detection assays of nosocomial pathogens for better prognosis and initiation of antimicrobial therapy in patients. In this study, we present the development of molecular methods for the detection of six common nosocomial pathogens including Escherichia coli, Staphylococcus aureus, Streptococcus pneumoniae, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter spp. Conventional multiplex PCR and SYBR Green based real time PCR assays were performed using genus and species specific primers. Blind testing with 300 clinical samples was also carried out. The two assays were found to be... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conventional multiplex PCR; SYBR Green Real time PCR; Nosocomial Pathogens. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200006 |
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