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| Yan,Jian; Jiao,Yan; Li,Xinmin; Jiao,Feng; Beamer,Wesley G.; Rosen,Cliff J.; Gu,Weikuan. |
| Humans and guinea pigs are species which are unable to synthesize ascorbic acid (vitamin C) because, unlike rodents, they lack the enzyme L-gulonolactone oxidase (Gulo). Although the phenotype of lacking vitamin C in humans, named scurvy, has long been well known, information on the impact of lacking Gulo on the gene expression profiles of different tissues is still missing. This knowledge could improve our understanding of molecular pathways in which Gulo may be involved. Recently, we discovered a deletion that includes all 12 exons in the gene for Gulo in the sfx mouse, characterized by spontaneous bone fractures. We report here the initial analysis of the impact of the Gulo gene deletion on the murine gene expression profiles in the liver, femur and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spontaneous fracture; Bone; Gene expression; L-gulonolactone oxidase; Vitamin C. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300004 |
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| Jiao,Yan; Zhang,Jifei; Yan,Jian; Stuart,John; Gibson,Griffin; Lu,Lu; Willaims,Robert; Wang,Yong Jun; Gu,Weikuan. |
| The aim of this study was to test the hypothesis that hepatic vitamin C (VC) levels in VC deficient mice rescued with high doses of VC supplements still do not reach the optimal levels present in wild-type mice. For this, we used a mouse scurvy model (sfx) in which the L-gulonolactone oxidase gene (Gulo) is deleted. Six age- (6 weeks old) and gender- (female) matched wild-type (WT) and sfx mice (rescued by administering 500 mg of VC/L) were used as the control (WT) and treatment (MT) groups (n = 3 for each group), respectively. Total hepatic RNA was used in triplicate microarray assays for each group. EDGE software was used to identify differentially expressed genes and transcriptomic analysis was used to assess the potential genetic regulation of Gulo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gene expression; L-gulonolactone oxidase; Liver; Oxidative stress; Vitamin C. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300004 |
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