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| Sathiyaraj,Gayathri; Srinivasan,Sathiyaraj; Kim,Ho-Bin; Subramaniyam,Sathiyamoorthy; Lee,Ok Ran; Kim,Yeon-Ju; Yang,Deok Chun. |
| Cylindrocarpon destructans isolated from ginseng field was found to produce pectinolytic enzymes. A Taguchi's orthogonal array experimental design was applied to optimize the preliminary production of polygalacturonase (PG) and pectin lyase (PL) using submerged culture condition. This method was applied to evaluate the significant parameters for the production of enzymes. The process variables were pH, pectin concentration, incubation time and temperature. Optimization of process parameters resulted in high levels of enzyme (PG and PL) production after ten days of incubation at a pH of 5.0 at 25°C in the presence of 1.5% pectin. Among different nitrogen sources, urea and peptone showed high production of PG and PL, respectively. The enzyme production and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C. destructans; Polygalacturonase; Pectin lyases; Ginseng. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200048 |
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| Quan,Lin-Hu; Piao,Jin-Ying; Min,Jin-Woo; Yang,Dong-Uk; Lee,Hee Nyeong; Yang,Deok Chun. |
| About 40 different types of ginsenoside (ginseng saponin), a major pharmacological component of ginseng, have been identified along with their physiological activities. Among these, compound K has been reported to prevent the development of and the metastasis of cancer by blocking the formation of tumors and suppressing the invasion of cancerous cells. In this study, ginsenoside Rb1 was converted into compound K via interaction with the enzyme secreted by β-glucosidase active bacteria, Leuconostoc citreum LH1, extracted from kimchi. The optimum time for the conversion of Rb1 to compound K was about 72 hrs at a constant pH of 6.0 and an optimum temperature of about 30ºC. Under optimal conditions, ginsenoside Rb1 was decomposed and converted into compound K... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bioconversion; Compound K; Ginsenoside Rb1; Leuconostoc citreum LH1. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300049 |
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