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	Provedor de dados Electron. J. Biotechnol. (1) Autor Chen,Dian Xu (1) Tang,Yue Yi (1) Wang,Chuan Tang (1) Wang,Xiu Zhen (1) Yu,Shan Lin (1) Yu,Shu Tao (1) Zhang,Jian Cheng (1) Mais... Palavra-chave Cotyledonary tissue (1) DNA extraction (1) Groundnut (1) PCR (1) Peanut (1) Tipo do documento Journal article (1) Ano 2010 (1) País Chile (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction Electron. J. Biotechnol. Yu,Shu Tao; Wang,Chuan Tang; Yu,Shan Lin; Wang,Xiu Zhen; Tang,Yue Yi; Chen,Dian Xu; Zhang,Jian Cheng. An efficient DNA extraction method was developed for peanut seed, where only 3-5 mg cotyledonary tissue was enough for more than 50 PCR reactions with a reaction volume of 15 μl. Both low copy number and high copy number DNA sequences were successfully amplified. Processing one seed sample only took about half an hour. Sampling had no significant effects on germination and development. The DNA extraction method makes it possible to identify transformants and conduct molecular marker studies prior to sowing, and thus may greatly hasten research progress. Tipo: Journal article Palavras-chave: Cotyledonary tissue; DNA extraction; Groundnut; PCR; Peanut. Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400012 Registros recuperados: 1 Primeira ... 1 ... Última

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