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Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor Electron. J. Biotechnol.
Sequeida,Álvaro; Tapia,Eduardo; Ortega,Marcelo; Zamora,Pablo; Castro,Álvaro; Montes,Christian; Zúñiga,Gustavo E; Prieto,Humberto.
Deschampsia antarctica (DA), the only species in the Gramineae family endemic to the Antarctic territory, is characterized by a combination of high levels of free endogenous phenylpropanoid compounds under normal in situ and in vitro growth conditions. In this article, we describe the design and use of a specific temporary immersion photobioreactor to produce both increased DA biomass and secondary metabolite accumulation by UV-B elicitation during cultivation. Three min-long immersions in an induction medium applied every 4 hrs at 14ºC ± 1 and 20/4 hrs light/darkness photoperiod increased DA biomass production over previous in vitro reports. Biomass duplication was obtained at day 10.7 of culturing, and maximum total phenolics and antioxidant activity...
Tipo: Journal article Palavras-chave: Deschampsia antarctica; Phenolic compounds; Temporary immersion bioreactor; UV-B elicitation.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400008
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Soluble carbohydrate content variation in Sanionia uncinata and Polytrichastrum alpinum, two Antarctic mosses with contrasting desiccation capacities Biol. Res.
Zúñiga-González,Paz; Zúñiga,Gustavo E; Pizarro,Marisol; Casanova-Katny,Angélica.
BACKGROUND: Cryptogamic vegetation dominates the ice-free areas along the Antarctic Peninsula. The two mosses Sanionia uncinata and Polytrichastrum alpinum inhabit soils with contrasting water availability. Sanionia uncinata grows in soil with continuous water supply, while P. alpinum grows in sandy, non-flooded soils. Desiccation and rehydration experiments were carried out to test for differences in the rate of water loss and uptake, with non-structural carbohydrates analysed to test their role in these processes. RESULTS: Individual plants of S. uncinata lost water 60 % faster than P. alpinum; however, clumps of S. uncinata took longer to dry than those of P. alpinum (11 vs. 5 h, respectively). In contrast, rehydration took less than 10 min for both...
Tipo: Journal article Palavras-chave: Antarctica; Antarctic vegetation; Bryophytes; Sugars.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100006
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Cold storage effects on oxidative stress of Red Globe table grape rachises Ciencia e Investigación Agraria
Campos-Vargas,Reinaldo; Zamora,Pablo; Contreras,Rodrigo; Köhler,Hans; Zúñiga,Gustavo E; Pérez-Donoso,Alonso; Defilippi,Bruno G.
Table grape (Vitis vinifera L.) quality includes the condition of both the berries and the rachis. In the present report, physiological parameters of Red Globe rachises from fully elongated inflorescences (RFEI) and from mature clusters were studied after storage at 0 or 20 °C for different durations. To understand changes in rachis physiology as a result of changes in temperature conditions and storage time, the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were measured. In addition, hydrogen peroxide content, membrane lipoperoxidation (TBARS), total phenolic compounds and antioxidant capacity (FRAP) were assayed. TBARS was higher in mature rachises than in RFEI. This parameter remained constant throughout...
Tipo: Journal article Palavras-chave: Fruit quality; Rachis; Storage; Table grape; Vitis vinifera L.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000100007
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