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| ZHANG,YONG; HAN,TAO; ZHU,QING; ZHANG,WEI; BAO,WEI; FU,HAI-JING; YANG,JIE; HUANG,XIAO-JUN; WEI,JUN-XIA; MENG,YAN-LING; ZHAO,JING; CAO,YUN-XIN; JIA,LIN-TAO; YANG,AN-GANG. |
| Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein that mediates both NADH-oxidizing and caspase-independent apoptosis. Further, the proapoptotic activity of AIF is located in the C-terminus of AIF, although the precise minimum sequence responsible for apoptosis induction remains to be investigated. In the present study, we generated two truncated AIFs, AIFΔ1-480-FLAG, which is a FLAG-tagged C-terminal peptide comprising amino acids from 481 to 613, and AIF360-480 containing amino acids from 360 to 480 of AIF. We used confocal microscopy to demonstrate that both the truncated proteins are expressed and located in the cytoplasm of transfected cells. AIFΔ1-480 but not AIF360-480 induces apoptosis in transfected cells. We also... |
| Tipo: Journal article |
Palavras-chave: Apoptosis inducing factor (AIF); Apoptosis; Cytochrome c; Mitochondria; HER2. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200014 |
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