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Secondary structure of nrDNA Internal Transcribed Spacers as a useful tool to align highly divergent species in phylogenetic studies Genet. Mol. Biol.
Giudicelli,Giovanna C.; Mäder,Geraldo; Silva-Arias,Gustavo A.; Zamberlan,Priscilla M.; Bonatto,Sandro L.; Freitas,Loreta B..
Abstract Recently, it has been suggested that internal transcribed spacer (ITS) sequences are under selective constraints to preserve their secondary structure. Here, we investigate the patterns of the ITS nucleotide and secondary structure conservation across the Passiflora L. genus to evaluate the potential use of secondary structure data as a helpful tool for the alignment in taxonomically complex genera. Considering the frequent use of ITS, this study also presents a perspective on future analyses in other plant groups. The ITS1 and ITS2 sequences presented significant differences for mean values of the lowest energy state (LES) and for number of hairpins in different Passiflora subgenera. Statistical analyses for the subgenera separately support...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Passiflora; Lowest energy state; Hairpins; ITS1; ITS2.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200191
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The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae) Genet. Mol. Biol.
Mäder,Geraldo; Zamberlan,Priscilla M.; Fagundes,Nelson J.R.; Magnus,Tielli; Salzano,Francisco M.; Bonatto,Sandro L.; Freitas,Loreta B..
The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic diversity; Intraspecific variability; ITS; Passiflora; Phylogeography.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018
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Phylogeny, biogeography and divergence times in Passiflora (Passifloraceae) Genet. Mol. Biol.
Muschner,Valéria C.; Zamberlan,Priscilla M.; Bonatto,Sandro L.; Freitas,Loreta B..
As part of a long-term investigation on the evolution of Passiflora L., we investigated the divergence ages of the genus and diversification of its subgenera, relating them with biogeographical and/or historical events, and other characteristics of this taxon. The main aim of the present work was to evaluate the biogeographic distribution of this genus to better understand its evolutionary history. This is the first time that representatives from South American and Old World Passifloraceae genera have been studied as a group comprising a total of 106 widely distributed species, with representative samples of the four suggested subgenera. Seven DNA regions were studied, comprising 7,431 nucleotides from plastidial, mitochondrial and nuclear genomes....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biogeography; Molecular phylogenetics analysis; Passionflowers; Plant evolution; Taxonomic classification.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600019
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