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	Provedor de dados BJMBR (2) Autor Lopes,M.T.P. (2) Salas,C.E. (2) Zanini,M.S. (2) Augusto,C. (1) Barouni,A.S. (1) Carneiro,A.M.D. (1) Genelhu,M.S. (1) Queiroz,M.V.N.P. (1) Veloso,I.F. (1) Mais... Palavra-chave Antibiotic resistance (1) Bacille Calmette-Guérin (BCG) (1) C. candamarcensis (1) Cysteine proteinase (1) DNA extraction (1) Lymphadenopathy (1) Polymerase chain reaction (1) Proteinase K (1) Vaccination (1) Mais... Tipo do documento Info:eu-repo/semantics/other (2) Ano 2004 (1) 1998 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Use of a cysteine proteinase from Carica candamarcensis as a protective agent during DNA extraction BJMBR Genelhu,M.S.; Zanini,M.S.; Veloso,I.F.; Carneiro,A.M.D.; Lopes,M.T.P.; Salas,C.E.. We describe the use of a plant cysteine proteinase isolated from latex of Carica candamarcensis as a protective agent during isolation of bacterial DNA following growth in culture of these cells. Between 100 to 720 units of proteinase (1 µg = 6 units) afforded good DNA protection when incubated with various kinds of microorganisms. Agarose gel electrophoresis showed that the resulting DNA was similar in size to DNA preparations obtained by treatment with proteinase K. The viability of the resulting material was checked by PCR amplification using species-specific primers. After standing at room temperature (25oC) for 35 days, the enzyme lost 10% of its initial activity. The enzyme stability and good yield of DNA suggest the use of this proteinase as an... Tipo: Info:eu-repo/semantics/other Palavras-chave: Cysteine proteinase; Proteinase K; C. candamarcensis; DNA extraction. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900005 BCG lymphadenopathy detected in a BCG-vaccinated infant BJMBR Barouni,A.S.; Augusto,C.; Queiroz,M.V.N.P.; Lopes,M.T.P.; Zanini,M.S.; Salas,C.E.. Large-scale vaccination with BCG, the live attenuated strain of Mycobacterium bovis, is being adopted around the world, although sporadic complications have occurred after the procedure. Lymphadenopathy is not uncommon especially in babies under one year (0.73% of vaccinated infants), but the swelling subsides within 2 months in most cases, with no medical or surgical treatment. Brazil adopted BCG vaccination program earlier in the seventies and by 1995 more than 96% of the infant population received this immunization. We report here the occurrence of lymphadenopathy in a two-year-old child vaccinated with the Brazilian BCG strain. The diagnosis was made using a lymph node biopsy and intestinal aspirates that yielded a positive mycobacterial culture. The... Tipo: Info:eu-repo/semantics/other Palavras-chave: Bacille Calmette-Guérin (BCG); Vaccination; Polymerase chain reaction; Lymphadenopathy; Antibiotic resistance. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000500011 Registros recuperados: 2 Primeira ... 1 ... Última

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