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| Pereira,Marli Aparecida dos Santos; Arantes,Márcia Alessandra; Molinari,Sonia Lucy; Zanoni,Jacqueline Nelisis; Miranda-Neto,Marcílio Hubner de. |
| The objective of our work was to verify, during an experimental period of 210 days, the possible effects of the ethanol on the morphology and density of the NADH-diaforase myenteric neurons in the jejunum of alcoholic rats. We used 10 animals (Rattus norvegicus) separated in 2 groups: the controls (n=5), that received a normal proteic diet (22%) and water ad libitum; the alcoholic, that received NUVILAB normal proteic chow (22%) and sugar cane brandy diluted at 30 Gay Lussac (30 v/v). The jejunum segment was collected and submitted to the histochemical technique to stain the nervous cells and, then, to the elaboration of membrane whole mounts. The observation through light microscopy showed that there are no expressive morphologic differences between the... |
| Tipo: Journal article |
Palavras-chave: Jejunum; Myenteric neurons; Rats; Alcoholism. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400016 |
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| Pereira,Marli Aparecida dos Santos; Liberati,Ana Paula; Souza,Ivan Domicio da Silva; Zanoni,Jacqueline Nelisis. |
| The objective of this work was to evaluate the overall myenteric neurons population from the duodenum of adult streptozotocin-induced diabetic rats supplemented with ascorbic acid (AA), a potent antioxidant. Fifteen 90-day-old rats were divided in groups: control (C), diabetic (D), diabetic treated with ascorbic acid (DA). After 120 days of experimental period duodenums were resected and processed as whole-mount preparations according to Giemsa's technique, which allowed us to evaluate neuronal density in an area of 8.96 mm² and measure the area of 500 neuronal cell bodies per group. It was observed a 32.55% reduction in neuronal density of group D when compared to group C (p<0.05). The density of spared neurons in group DA, in relation to group D, was... |
| Tipo: Journal article |
Palavras-chave: Ascorbic acid; Diabetes mellitus; Duodenum; Oxidative stress. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200017 |
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| Pereira,Marli Aparecida dos Santos; Miranda Neto,Marcílio Hubner; Arantes,Márcia Alessandra; Molinari,Sonia Lucy; Pereira,Priscila; Zanoni,Jacqueline Nelisis. |
| Morphometric and quantitative analyses were accomplished to study the effects of the consumption of alcohol above the size and density of the myenteric neurons of cecum of rats. Ten rats with 90 days were divided in the groups: control (C), and alcoholic (A). After 120 days of treatment with ethanol the cecum of both groups were collected, submitted it prepared of membranes that after carried out to Giemsa´s technique, permitted to evaluate the neuronal density, in an area of 13.44mm², and measure the cell body area of 300 neurons by group. The alcoholic rats presented an increase in the number of small neurons and a reduction of the big and medium neurons. The neuronal density verified in alcoholic rats was significantly reduced regarding the controls... |
| Tipo: Journal article |
Palavras-chave: Alcoholism; Cecum; Myenteric neurons. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000300001 |
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