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Paula,Daniella A. J.; Andrade,Estefânia S.; Murgas,Luis D. S.; Felizardo,Viviane O.; Winkaler,Elissandra U.; Zeviani,Walmes; Freitas,Rilke T. F.. |
This study investigated the addition of antioxidants vitamin E and reduced glutathione on curimba (Prochilodus lineatus) semen cryopreservation and compared sodium bicarbonate solution and distilled water as activators. The experiment was conducted at the environmental station of CEMIG, in Itutinga-MG, Brazil, between December/2009 and January/2010. Semen samples (n = 7) with semen motility above 80% were diluted in cryoprotectant solutions composed of 10% methanol, 15% lactose and containing different concentrations of antioxidants: 50 (VE50), 100 (VE100) and 250 (VE250) µM of vitamin E, and 0.5 (RG0.5), 1.0 (RG1.0) and 1.5 (RG1.5) mM of reduced glutathione. A solution without antioxidants was used as a control. The semen was diluted at a ratio of 1:4... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; Fertilization; Freezing; Reproduction; Sperm motility. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252012000300020 |
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