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| Chu,Yanchen; Fang,Yuan; Chi,Jingwei; Li,Jing; Zhang,Dongyang; Zou,Yunwen; Wang,Zhijie. |
| Osteosarcoma (OS) has a high incidence, malignity, and frequency of recurrence and metastasis. In this study, we aimed to explore the potential anti-cancer effects of Astragalus polysaccharides (APS) on human OS MG63 cells as well as underlying mechanisms. Viability of MG63 cells was assessed by CCK-8 assay to determine the adequate concentration of APS. Then, effects of APS on MG63 cell proliferation, cell cycle distribution, apoptosis, and migration and invasion were analyzed by BrdU incorporation, PI staining, flow cytometry, and transwell assays, respectively. The expression levels of proteins involved in these physiological processes were assessed by western blot analysis. Afterwards, miR-133a level in APS-treated cells was determined by qRT-PCR, and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Osteosarcoma; Astragalus polysaccharides; Anti-tumor; MicroRNA-133a; JNK. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200610 |
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