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	Provedor de dados Electron. J. Biotechnol. (3) Autor Zhang,Guangya (3) Fang,Baishan (2) Li,Wei (2) Ng,I-Son (2) Ge,Huihua (1) Yu,Jincong (1) Mais... Palavra-chave 1 (2) 3-propanediol oxidoreductase codon optimization enzymatic properties Escherichia coli overlap extension PCR (1) 3-propanediol oxidoreductase elastin-like polypeptides Escherichia coli fusion protein non-chromatographic purification (1) Amino acid composition (1) Optimum temperature (1) Support vector machine (1) Uniform design (1) Xylanase (1) Mais... Tipo do documento Journal article (3) Ano 2012 (1) 2011 (2) País Chile (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Codon optimization of 1,3-propanediol oxidoreductase expression in Escherichia coli and enzymatic properties Electron. J. Biotechnol. Li,Wei; Ng,I-Son; Fang,Baishan; Yu,Jincong; Zhang,Guangya. The gene dhaT from Klebsiella pneumoniae encoding 1,3-propanediol oxidoreductase (PDOR) was de novo synthesized by splicing overlap extension polymerase chain reaction (SOE-PCR) primarily according to Escherichia coli’s codon usage, as well as mRNA secondary structure. After optimization, Codon Adaptation Index (CAI) value was improved from 0.75 to 0.83, meanwhile energy of mRNA secondary structure was increased from -400.1 to -86.8 kcal/mol. This synthetic DNA was under control by phage T7 promoter in the expression vector pET-15b and transformed into the E. coli BL21 (DE3) strain. Inducers such as isopropyl β-D-thiogalactoside (IPTG) and lactose were compared by activity at different inducing time. The activity of PDOR after codon optimized was... Tipo: Journal article Palavras-chave: 1; 3-propanediol oxidoreductase codon optimization enzymatic properties Escherichia coli overlap extension PCR. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400007 Prediction of xylanase optimal temperature by support vector regression Electron. J. Biotechnol. Zhang,Guangya; Ge,Huihua. Background: Support vector machine (SVM), a novel powerful machine learning technology, was used to develop the non-linear quantitative structure-property relationship (QSPR) model of the G/11 xylanase based on the amino acid composition. The uniform design (UD) method was applied to optimize the running parameters of SVM for the first time. Results: Results showed that the predicted optimum temperature of leave-one-out (LOO) cross-validation fitted the experimental optimum temperature very well, when the running parameter C, ξ, and γ was 50, 0.001 and 1.5, respectively. The average root-mean-square errors (RMSE) of the LOO cross-validation were 9.53ºC, while the RMSE of the back propagation neural network (BPNN), was 11.55ºC. The... Tipo: Journal article Palavras-chave: Amino acid composition; Optimum temperature; Support vector machine; Uniform design; Xylanase. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100007 Expression and non-chromatographic purification of 1,3-propanediol oxidoreductase in Escherichia coli Electron. J. Biotechnol. Li,Wei; Ng,I-Son; Fang,Baishan; Zhang,Guangya. The gene dhaT from Klebsiella pneumoniae encodes 1,3-propanediol oxidoreductase (PDOR). Thermally responsive elastin-like polypeptides (ELPs) was used as a fusion tag to purify the proteins (PDOR). The ELP gene was attached to dhaT and ligated into the pET-22b vector. Different NaCl concentrations were employed to decrease the transition temperature (Tt) which was diminished as salt concentration increased. The optimal final concentration of NaCl was 1 M and the corresponding Tt was 39.5ºC. Enzymatic assays were determined via every step for purification of fusion PDOR. PDOR showed good stability during the purification process, the specific activity in the first and second round of inverse transition cycling (ITC) was 276.1 ± 13.3 and 213.3 ± 10.8 U/mg,... Tipo: Journal article Palavras-chave: 1; 3-propanediol oxidoreductase elastin-like polypeptides Escherichia coli fusion protein non-chromatographic purification. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600010 Registros recuperados: 3 Primeira ... 1 ... Última

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